A Single Immunization with Spike-Functionalized Ferritin Vaccines Elicits Neutralizing Antibody Responses against SARS-CoV-2 in Mice
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SciScore for 10.1101/2020.08.28.272518: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable Mouse immunizations: Balb/c female mice (6-8 weeks old) were procured from The Jackson Laboratory. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources In-house made primary antibodies CR3022, COVA2-15, and CB6 (approximate concentrations 0.8 – 1.3 mg/mL) were added at a 1:10,000 dilution in PBST. COVA2-15suggested: NoneCB6suggested: NoneSecondary antibody was added at 1:10,000 (abcam ab6759, rabbit anti-human IgG H&L HRP) in 5% milk / PBST and incubated for 1 h at room temperature. anti-human IgGsuggested: NoneFor … SciScore for 10.1101/2020.08.28.272518: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable Mouse immunizations: Balb/c female mice (6-8 weeks old) were procured from The Jackson Laboratory. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources In-house made primary antibodies CR3022, COVA2-15, and CB6 (approximate concentrations 0.8 – 1.3 mg/mL) were added at a 1:10,000 dilution in PBST. COVA2-15suggested: NoneCB6suggested: NoneSecondary antibody was added at 1:10,000 (abcam ab6759, rabbit anti-human IgG H&L HRP) in 5% milk / PBST and incubated for 1 h at room temperature. anti-human IgGsuggested: NoneFor purified mAbs and ACE2-Fc, Direct-Blot HRP anti-human IgG1 Fc antibody (Biolegend 410604) was added at a 1:10,000 dilution in diluent buffer for 1 h at room temperature. anti-human IgG1suggested: (BioLegend Cat# 410604, RRID:AB_2749913)CCP samples were obtained from donors who had a clinical diagnosis of COVID-19 and either a positive RT-PCR or SARS-CoV-2 antibody test. SARS-CoV-2suggested: NoneExperimental Models: Cell Lines Sentences Resources 2 pseudotyped lentivirus production and viral neutralization assays: SARS-CoV-2 spike pseudotyped lentivirus was produced in HEK293T cells via calcium phosphate transfection. HEK293Tsuggested: NCBI_Iran Cat# C498, RRID:CVCL_0063)Experimental Models: Organisms/Strains Sentences Resources Mouse immunizations: Balb/c female mice (6-8 weeks old) were procured from The Jackson Laboratory. Balb/csuggested: NoneSoftware and Algorithms Sentences Resources Dots were quantified using the gel analysis protocol in Fiji (ImageJ) and curves were fit using a linear regression in GraphPad Prism 8.4.1. SEC-MALS of SARS-CoV-2 antigens: SEC-MALS was performed on an Agilent 1260 Infinity II HPLC with Wyatt detectors for light scattering (miniDAWN) and refractive index (Optilab) Fijisuggested: (Fiji, RRID:SCR_002285)ImageJsuggested: (ImageJ, RRID:SCR_003070)GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Molecular weights were determined using ASTRA 7.3.2 (Wyatt Technologies). ASTRAsuggested: (ASTRA, RRID:SCR_016255)Single-particle image processing and 3D reconstruction: All movie stacks were first imported into Relion 3.0.6 (70) for image processing. Relionsuggested: (RELION, RRID:SCR_016274)Motion-correction was performed with MotionCor2 1.3.2 (71) and the contrast transfer function was determined with CTFFIND4 4.1.13 (72). MotionCor2suggested: (MotionCor2, RRID:SCR_016499)The initial model was built in cryoSPARC (74) using the ab-initio reconstruction option with octahedral symmetry applied. cryoSPARCsuggested: (cryoSPARC, RRID:SCR_016501)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: We found the following clinical trial numbers in your paper:
Identifier Status Title NCT03186781 Completed Influenza HA Ferritin Vaccine, Alone or in Prime-Boost Regim… NCT03814720 Active, not recruiting Dose, Safety, Tolerability and Immunogenicity of an Influenz… Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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