Topography, Spike Dynamics, and Nanomechanics of Individual Native SARS-CoV-2 Virions
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SciScore for 10.1101/2020.09.17.302380: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources The anti-spike glycoprotein antibody-coated surfaces were stored under PBS for up to 5 days at 4 °C. anti-spike glycoproteinsuggested: NoneHandling virion sample in the AFM: An aliquot (20 μl) of purified SARS-CoV-2 sample was pipetted onto the anti-spike antibody-coated substrate surface and incubated at 37 °C for 30 minutes. anti-spike antibody-coated substrate surfacesuggested: NoneExperimental Models: Cell Lines Sentences Resources Sample preparation: … SciScore for 10.1101/2020.09.17.302380: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources The anti-spike glycoprotein antibody-coated surfaces were stored under PBS for up to 5 days at 4 °C. anti-spike glycoproteinsuggested: NoneHandling virion sample in the AFM: An aliquot (20 μl) of purified SARS-CoV-2 sample was pipetted onto the anti-spike antibody-coated substrate surface and incubated at 37 °C for 30 minutes. anti-spike antibody-coated substrate surfacesuggested: NoneExperimental Models: Cell Lines Sentences Resources Sample preparation: SARS-CoV-2 was isolated from the oropharyngeal swab of a laboratory-confirmed COVID-19 patient in Hungary and passed two times in VeroE6 cell line (European Collection of Authenticated Cell Culture, Salisbury, U.K.) in Dulbecco’s Modified Eagle’s Medium (Lonza, Basel, Switzerland) supplemented with 5% fetal bovine serum (EuroClone, Pero, Italy) and Cell Culture Guard (PanReac AppliChem, Darmstadt, Germany). VeroE6suggested: JCRB Cat# JCRB1819, RRID:CVCL_YQ49)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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