Topography, Spike Dynamics, and Nanomechanics of Individual Native SARS-CoV-2 Virions

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1. Version published to 10.1021/acs.nanolett.0c04465

   Jan 21, 2021
2. 

   ScreenIT

   Sep 21, 2020

   SciScore for 10.1101/2020.09.17.302380: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   The anti-spike glycoprotein antibody-coated surfaces were stored under PBS for up to 5 days at 4 °C.	anti-spike glycoprotein suggested: None
   Handling virion sample in the AFM: An aliquot (20 μl) of purified SARS-CoV-2 sample was pipetted onto the anti-spike antibody-coated substrate surface and incubated at 37 °C for 30 minutes.	anti-spike antibody-coated substrate surface suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   Sample preparation: …

   SciScore for 10.1101/2020.09.17.302380: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   The anti-spike glycoprotein antibody-coated surfaces were stored under PBS for up to 5 days at 4 °C.	anti-spike glycoprotein suggested: None
   Handling virion sample in the AFM: An aliquot (20 μl) of purified SARS-CoV-2 sample was pipetted onto the anti-spike antibody-coated substrate surface and incubated at 37 °C for 30 minutes.	anti-spike antibody-coated substrate surface suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   Sample preparation: SARS-CoV-2 was isolated from the oropharyngeal swab of a laboratory-confirmed COVID-19 patient in Hungary and passed two times in VeroE6 cell line (European Collection of Authenticated Cell Culture, Salisbury, U.K.) in Dulbecco’s Modified Eagle’s Medium (Lonza, Basel, Switzerland) supplemented with 5% fetal bovine serum (EuroClone, Pero, Italy) and Cell Culture Guard (PanReac AppliChem, Darmstadt, Germany).	VeroE6 suggested: JCRB Cat# JCRB1819, RRID:CVCL_YQ49)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Read the original source
3. 

   Version published to 10.1101/2020.09.17.302380v1 on bioRxiv

   Sep 17, 2020