Combinatorial Approach with Mass Spectrometry and Lectin Microarray Dissected Site-Specific Glycostem and Glycoleaf Features of the Virion-Derived Spike Protein of Ancestral and γ Variant SARS-CoV-2 Strains

  1. Takahiro Hiono
  2. Hiroaki Sakaue
  3. Azusa Tomioka
  4. Hiroyuki Kaji
  5. Michihito Sasaki
  6. Yasuko Orba
  7. Hirofumi Sawa
  8. Atsushi Kuno

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Abstract

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  1. Version published to 10.1021/acs.jproteome.3c00874
  2. ScreenIT

    SciScore for 10.1101/2021.04.10.439300: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board Statementnot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    Antibodies: Rabbit monoclonal anti-SARS-CoV-2 spike S1 antibody, 007 (Cat# 40150-R007), and mouse monoclonal anti-SARS-CoV-2 spike neutralizing antibody, 43 (Cat# 40591-V05H1), were purchased from Sino Biological (Beijing, China).
    Antibodies: Rabbit monoclonal anti-SARS-CoV-2 spike S1 antibody
    suggested: None
    anti-SARS-CoV-2 spike S1
    suggested: None
    anti-SARS-CoV-2 spike neutralizing antibody , 43
    suggested: None
    These antibodies were biotinylated using the Biotin Labeling Kit-NH2 (Dojindo, Kumamoto, Japan)
    Biotin Labeling Kit-NH2 ( Dojindo , Kumamoto , Japan)
    suggested: None
    Experimental Models: Cell Lines
    SentencesResources
    VeroE6/TMPRSS2 cells were established in our laboratory as described previously23.
    VeroE6/TMPRSS2
    suggested: JCRB Cat# JCRB1819, RRID:CVCL_YQ49)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2021.04.10.439300v1 on bioRxiv