Design of SARS-CoV-2 PLpro Inhibitors for COVID-19 Antiviral Therapy Leveraging Binding Cooperativity
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SciScore for 10.1101/2021.02.13.431008: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Cell Culture and cytotoxicity: African green monkey kidney epithelial cells Vero E6 (ATCC# CRL-1586) were cultured in DMEM supplemented with 10% fetal bovine serum (Gibco), 100 units of penicillin and 100 µg/mL streptomycin (Invitrogen). Vero E6suggested: RRID:CVCL_XD71)Human alveolar epithelial cell line (A549) that stably express hACE2 are from BEI Resources (NR-53821). A549suggested: NoneA549-hACE2 cells were seeded 1.5×105 … SciScore for 10.1101/2021.02.13.431008: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Cell Culture and cytotoxicity: African green monkey kidney epithelial cells Vero E6 (ATCC# CRL-1586) were cultured in DMEM supplemented with 10% fetal bovine serum (Gibco), 100 units of penicillin and 100 µg/mL streptomycin (Invitrogen). Vero E6suggested: RRID:CVCL_XD71)Human alveolar epithelial cell line (A549) that stably express hACE2 are from BEI Resources (NR-53821). A549suggested: NoneA549-hACE2 cells were seeded 1.5×105 cells/well in DMEM complete into 24-well plates (0.5 mL/well) then incubated for 16 hours at 37 °C and 5% CO2. A549-hACE2suggested: NoneSoftware and Algorithms Sentences Resources Bioactive Library) and ChemDiv (a 10,000-compound SMART library subset) SMARTsuggested: (SMART, RRID:SCR_005026)Rigid body refinement followed by iterative rounds of restrained refinement and model building were performed with CCP4i modules Refmac559 and Coot. Cootsuggested: (Coot, RRID:SCR_014222)EC50 values were determined by fitting the dose-response curves with four-parameter logistic regression in Prism GraphPad (version 8.1.2). Prismsuggested: (PRISM, RRID:SCR_005375)GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)Statistical analysis: GraphPad Prism 8 software package (GraphPad Software, USA) was used to perform statistical analysis. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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