Regional Replacement of SARS-CoV-2 Variant Omicron BA.1 with BA.2 as Observed through Wastewater Surveillance
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SciScore for 10.1101/2022.04.22.22274160: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Field Sample Permit: Variants present in regional clinical specimens: The 7-d, centered, rolling average proportion of clinical specimens sequenced from California classified as Omicon BA.1* and BA.2* as a function of specimen collection data were acquired from GISAID3,4 on 21 April 2022. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
Software and Algorithms Sentences Resources Assays were developed in silico using Primer3Plus (https://primer3plus.com/) (Table S2). Primer3Plussuggested: (Primer3Plus, RRID:SCR_003081)Primers and probe sequences (Table 1) were screened for specificity in silico using NCBI BLAST, … SciScore for 10.1101/2022.04.22.22274160: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Field Sample Permit: Variants present in regional clinical specimens: The 7-d, centered, rolling average proportion of clinical specimens sequenced from California classified as Omicon BA.1* and BA.2* as a function of specimen collection data were acquired from GISAID3,4 on 21 April 2022. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
Software and Algorithms Sentences Resources Assays were developed in silico using Primer3Plus (https://primer3plus.com/) (Table S2). Primer3Plussuggested: (Primer3Plus, RRID:SCR_003081)Primers and probe sequences (Table 1) were screened for specificity in silico using NCBI BLAST, and then tested in vitro against various respiratory viruses and gRNA from wild-type (WT) SARS-CoV-2 and its variants (see SI). NCBI BLASTsuggested: (NCBI BLAST, RRID:SCR_004870)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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