Rational Design and Development of SARS-CoV-2 Serological Diagnostics by Immunoprecipitation-Targeted Proteomics
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SciScore for 10.1101/2021.10.25.21265408: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
No key resources detected.
Results from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:Limitations of such assays included semi-quantitative measurements due to the lack of “gold” standards, potential cross-reactivity, and inability to distinguish between antibody subclasses. Since reference samples with the standardized amounts of anti-pathogen antibodies or recombinant monoclonal antibodies are typically not available at the early stages of the …
SciScore for 10.1101/2021.10.25.21265408: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
No key resources detected.
Results from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:Limitations of such assays included semi-quantitative measurements due to the lack of “gold” standards, potential cross-reactivity, and inability to distinguish between antibody subclasses. Since reference samples with the standardized amounts of anti-pathogen antibodies or recombinant monoclonal antibodies are typically not available at the early stages of the novel pathogen epidemics, different laboratories calibrate serology tests with different convalescent serum samples obtained from the recovered patients. Lack of a single reference standard for assay calibration limits inter-laboratory and international standardization of serological tests, and is a recognized limitation. As a result of cross-reactivity, diagnostic specificity of serological antibody tests may not be sufficiently high to enable screening of the general asymptomatic populations for the acquired immunity against low-prevalence infectious diseases, such as COVID-19. For instance, 95% diagnostic specificity of COVID-19 serological tests36,37 and 0.3% prevalence in early 2020 in Canada would account for 5% positive predictive value. Interestingly, 90% positive predictive value at such low prevalence could only be achieved with a superior test with 99.97% diagnostic specificity. PCR and RT-PCR are undoubtedly the techniques with unprecedented analytical sensitivity. Sensitivity of protein assays, however, could be leveraged by the presence of numerous analyte copies (for example, ∼1,000 copies of NCAP_SARS2 ...
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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