Detecting Intact Virus Using Exogenous Oligonucleotide Labels

  1. Thomas R. Carey
  2. Molly Kozminsky
  3. Jennifer Hall
  4. Valerie Vargas-Zapata
  5. Kristina Geiger
  6. Laurent Coscoy
  7. Lydia L. Sohn

Reviewed by

Read the full article See related articles

Listed in

Log in to save this article

Abstract

No abstract available

Article activity feed

  1. Version published to 10.1021/acs.analchem.2c00835
  2. ScreenIT

    SciScore for 10.1101/2021.03.23.21254201: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board Statementnot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Experimental Models: Cell Lines
    SentencesResources
    Cell culture for lentivirus production: 293T cells were obtained from the UC Berkeley Cell Culture Facility.
    293T
    suggested: NCBI_Iran Cat# C498, RRID:CVCL_0063)
    . 293T-hACE2 cells were obtained from Jesse D.
    293T-hACE2
    suggested: None
    Software and Algorithms
    SentencesResources
    Mean fluorescent intensities of beads in each image were determined using the ‘Analyze Particles’ tool in ImageJ (NIH). qPCR: Samples suspended in nuclease-free water were mixed with qPCR master mix (Applied Biosystems PowerUp SYBR Green) and primers (Table S1; 50 nM final concentration; IDT).
    ImageJ
    suggested: (ImageJ, RRID:SCR_003070)
    Cells were analyzed by flow cytometry (Becton Dickinson LSRFortessa) using the FITC channel to detect ZsGreen and the data analyzed in FlowJo (v.10).
    FlowJo
    suggested: (FlowJo, RRID:SCR_008520)
    All statistical analysis and plotting were performed with GraphPad Prism 9.0.2.
    GraphPad Prism
    suggested: (GraphPad Prism, RRID:SCR_002798)

    Results from OddPub: Thank you for sharing your data.

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a protocol registration statement.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2021.03.23.21254201v1 on medRxiv