Rapid Screening of COVID-19 Directly from Clinical Nasopharyngeal Swabs Using the MasSpec Pen
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Abstract
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SciScore for 10.1101/2021.05.14.21257006: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics IRB: Approval from Institutional Review Board (IRB) was received for the study (protocol number 31573020.9.0000.5514, approved from May 29, 2020). Sex as a biological variable not detected. Randomization For Classifier 1, the data was randomly split into a training set (2/3 of data, n=97) and a validation set (1/3 of data, n=48). Blinding not detected. Power Analysis not detected. Table 2: Resources
No key resources detected.
Results from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:This study has a few limitations. Concerning clinical samples, the swabs for MS and PCR analysis were collected …
SciScore for 10.1101/2021.05.14.21257006: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics IRB: Approval from Institutional Review Board (IRB) was received for the study (protocol number 31573020.9.0000.5514, approved from May 29, 2020). Sex as a biological variable not detected. Randomization For Classifier 1, the data was randomly split into a training set (2/3 of data, n=97) and a validation set (1/3 of data, n=48). Blinding not detected. Power Analysis not detected. Table 2: Resources
No key resources detected.
Results from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:This study has a few limitations. Concerning clinical samples, the swabs for MS and PCR analysis were collected separately for hospitalized patients in our study, which could potentially lead to discrepancies in their diagnoses, especially considering the reported FNR of PCR analysis 45-47. Viral load information was also unavailable for the patients which prevented evaluation of a potential relationship between viral burden, molecular information, and diagnostic performance achieved. Heat-inactivation was also used in our study for all the clinical swabs, and thus, biosafety considerations in swab collection, storage, and inactivation steps are needed in future studies to facilitate sample collection and transport. Lastly, although our study was performed using a restricted population of individuals from Brazil, the promising results obtained warrants further investigation, and we expect that a larger cohort of patient samples including asymptomatic PCR positive patients and patients with other viral infections causing similar symptoms to COVID-19 will allow further refinement and validation of the classifiers for COVID-19 disease prediction using lipid information. In conclusion, the integration of a redesigned version of the disposable MasSpec Pen device provides a rapid MS-based screening method for COVID-19 disease directly from nasopharyngeal swabs. Modifications to the sampling unit and coupling to ESI enabled more effective and reproducible extraction and ionization o...
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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