Structural and Functional Characterization of SARS-CoV-2 RBD Domains Produced in Mammalian Cells
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SciScore for 10.1101/2021.02.23.432424: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Recombinant RBDs (Wuhan-Hu-1-isolate (MN908947)), either transiently expressed in HEK293 or stably expressed in CHO cells, were used (InVivo Biotech Services, Henningsdorf, Germany). CHOsuggested: CLS Cat# 603479/p746_CHO, RRID:CVCL_0213)Software and Algorithms Sentences Resources The spectra were processed in FlexAnalysis (Compass for flexSeries 2.0) using a smoothing step (5 cycles 0.15 Da) and baseline subtraction (TopHat). TopHatsuggest…SciScore for 10.1101/2021.02.23.432424: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Recombinant RBDs (Wuhan-Hu-1-isolate (MN908947)), either transiently expressed in HEK293 or stably expressed in CHO cells, were used (InVivo Biotech Services, Henningsdorf, Germany). CHOsuggested: CLS Cat# 603479/p746_CHO, RRID:CVCL_0213)Software and Algorithms Sentences Resources The spectra were processed in FlexAnalysis (Compass for flexSeries 2.0) using a smoothing step (5 cycles 0.15 Da) and baseline subtraction (TopHat). TopHatsuggested: (TopHat, RRID:SCR_013035)Peaks were picked using the SNAP2 algorithm up to 5,000 Da and the SNAP algorithm up to 10,000 Da. SNAPsuggested: (SNAP, RRID:SCR_007936)For interpretation and annotation of ISD spectra BioTools 3.2 SR7 was used (Bruker). BioToolssuggested: (MSU Mass Spectrometry Facility, RRID:SCR_012482)Glycan structures were assigned on the basis of the known MS/MS fragmentation patterns in negative-ion mode [24–26], elution order, and general glycobiological knowledge, with help of Glycoworkbench [27] and Glycomod software [28]. Glycomodsuggested: (GlycoMod, RRID:SCR_001602)In order to determine the linear correlation between the absorption values, the Pearson correlation was calculated using GraphPad Prism 9. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)GraphPad Prism 9 was used to plot log(dose) response curves (variable slope, four parameters) and to compute nonlinear fits which were utilized to calculate the half-maximal concentrations (EC50). GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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