Seroprevalence and dynamics of anti-SARS-CoV-2 antibody among healthcare workers following ChAdOx1 nCoV-19 vaccination

  1. Soma Sarkar
  2. Shantanab Das
  3. Kabita Choudhury
  4. Saibal Mukherjee
  5. Raghunath Chatterjee

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Abstract

Health care workers (HCWs) are in a higher risk of acquiring the disease owing to their regular contact with the patients. The aim of this study is to evaluate the seroprevalence among HCWs pre- and post-vaccination. The serological assessment of anti-SARS-CoV-2 antibody was conducted in pre- and post-vaccination of first or both doses of the ChAdOx1 nCoV-19 vaccine and followed up to 8 months for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and antibody titre. The neutralising antibody was positively correlated with IgG and total antibody. IgG was significantly decreased after 4–6 months post-infection. Almost all HCWs developed IgG after 2 doses of vaccine with comparable IgG to that of the infected HCWs. A follow-up of 6 to 8 months post vaccination showed a significant drop in antibody titre, while 56% of them didn't show a detectable level of IgG, suggesting the need for a booster dose. Around 21% of the vaccinated HCWs with significantly low antibody titre were infected with the SARS-CoV-2, but a majority of them showed mild symptoms and recovered in home isolation without any O 2 support. We noticed the effectiveness of the ChAdOx1 nCoV-19 vaccine as evident from the low rate of breakthrough infection with any severe symptoms.

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  1. Version published to 10.1017/s0950268822000747
  2. ScreenIT

    SciScore for 10.1101/2021.07.20.21260278: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    EthicsIACUC: Study setting: This cross-sectional study among the health care workers (HCWs) was executed at a tertiary care hospital, Kolkata after obtaining approval from the Institutional Ethics Committee,
    Consent: Among the 3,997 HCWs in the tertiary care center, 313 HCWs (11 Administrative staff, facility manager and clerical staff, 66 Junior doctors, 38 Medical officer, 81 Nursing staff, 24 Paramedical staff and research scientist, 93 Support staff, GDA, security, kitchen staff) were randomly selected and included in the study with their informed consent for serosurvey during November 2020 to January 2021.
    Sex as a biological variablenot detected.
    RandomizationAmong the 3,997 HCWs in the tertiary care center, 313 HCWs (11 Administrative staff, facility manager and clerical staff, 66 Junior doctors, 38 Medical officer, 81 Nursing staff, 24 Paramedical staff and research scientist, 93 Support staff, GDA, security, kitchen staff) were randomly selected and included in the study with their informed consent for serosurvey during November 2020 to January 2021.
    Blindingnot detected.
    Power Analysisnot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    A structured questionnaire comprising demographics, prior symptoms with their duration, prior COVID-19 test results, working location (in terms of working in COVID or Non-COVID unit), co-morbidities were collected prior to the blood collection for SARS-CoV-2 antibody testing.
    SARS-CoV-2
    suggested: None
    Serological analysis of IgG and total antibody (IgG, IgM and IgA) were performed using enhanced chemiluminescence technology by Vitros ECiQ (Ortho Clinical Diagnostics, New Jersey, US).
    total antibody ( IgG
    suggested: None
    IgA
    suggested: None
    When horseradish peroxidase (HRP) labelled murine monoclonal anti-Human IgG antibodies were added as conjugate, the conjugate interacted specifically to the antibody portion of antigen-antibody complex which is measured by a luminescent reaction.
    anti-Human IgG
    suggested: None

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a protocol registration statement.

    Results from scite Reference Check: We found no unreliable references.

    About SciScore

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  3. Version published to 10.1101/2021.07.20.21260278v1 on medRxiv