Pulmonary post-mortem findings in a series of COVID-19 cases from northern Italy: a two-centre descriptive study

  1. Luca Carsana
  2. Aurelio Sonzogni
  3. Ahmed Nasr
  4. Roberta Simona Rossi
  5. Alessandro Pellegrinelli
  6. Pietro Zerbi
  7. Roberto Rech
  8. Riccardo Colombo
  9. Spinello Antinori
  10. Mario Corbellino
  11. Massimo Galli
  12. Emanuele Catena
  13. Antonella Tosoni
  14. Andrea Gianatti
  15. Manuela Nebuloni

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Abstract

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  1. ScreenIT

    SciScore for 10.1101/2020.04.19.20054262: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board StatementIRB: This study is based on the histological analysis of post-mortem lung tissues from 38 cases who died for COVID-19 between February and March in two referral centres for the management of the COVID-19 outbreak in Northern Italy, Luigi Sacco Hospital, Milan, and Papa Giovanni XXIII, Bergamo (patient permission given by the Ethics Committees of the two hospitals to use personal and sensitive data for scientific research related to the disease was collected).
    Randomizationnot detected.
    BlindingHistological evaluation was performed blindly by two pathologists from each hospital, with expertise in the field.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.

    Table 2: Resources

    No key resources detected.

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  2. Version published to 10.1016/s1473-3099(20)30434-5
  3. ScreenIT

    SciScore for 10.1101/2020.04.19.20054262: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board StatementMETHODS This study is based on the histological analysis of post-mortem lung tissues from 38 cases wh for COVID-19 between February and March in two referral centres for the management of th COVID-19 outbreak in Northern Italy, Luigi Sacco Hospital, Milan, and Papa Giovanni XXI Bergamo (patient permission given by the Ethics Committees of the two hospitals to use pers and sensitive data for scientific research related to the disease was collected).Randomizationnot detected.BlindingHistological evaluation was performed blindly by two pathologists from each hospital, with expertise in the field.Power Analysisnot detected.Sex as a biological variableRESULTS Patients were 33 males and five females, average age of 69 years (range 32-86); the time that patients spent in the Subintensive/Intensive Care Unit ranged from 1 to 23 days (6.87 days).

    Table 2: Resources

    Antibodies
    SentencesResources
    Immunohistochemistry with anti CD61 antibody identified increased number of megakaryocytes in lung capillaries ( Figure 1 , panel C , inset) .
    anti CD61
    suggested: None
    Hematoxylin-Eosin , OM 10x Figure 2 Panel A and B: immunohistochemistry with antibody to CD68 ( A ) shows highly predo component of macrophages , focally with multinucleated cells feature.
    CD68
    suggested: None
    Immunohistochemist antibody to CD45 shows a few lymphocytes in interstitial septa ( CD68 and CD45 immunostaining , Hematoxylin counterstaining , OM x20) .
    CD45
    suggested: None

    Results from OddPub: We did not find a statement about open data. We also did not find a statement about open code. Researchers are encouraged to share open data when possible (see Nature blog).

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore is not a substitute for expert review. SciScore checks for the presence and correctness of RRIDs (research resource identifiers) in the manuscript, and detects sentences that appear to be missing RRIDs. SciScore also checks to make sure that rigor criteria are addressed by authors. It does this by detecting sentences that discuss criteria such as blinding or power analysis. SciScore does not guarantee that the rigor criteria that it detects are appropriate for the particular study. Instead it assists authors, editors, and reviewers by drawing attention to sections of the manuscript that contain or should contain various rigor criteria and key resources. For details on the results shown here, including references cited, please follow this link.

  4. Version published to 10.1101/2020.04.19.20054262v1 on medRxiv