Use of heated tobacco products, moderate alcohol drinking, and anti-SARS-CoV-2 IgG antibody titers after BNT162b2 vaccination among Japanese healthcare workers
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SciScore for 10.1101/2021.11.29.21267032: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Consent: Written informed consent was obtained from each participant.
IRB: The study design and procedure for data collection were approved by the ethical committee of each center, while those of pooling study were approved by that of the National Center for Global Health and Medicine (NCGM) (the approved number: NCGM-G-004233).Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis In post hoc analysis, we combined the categories of exclusive HNB tobacco users and dual users in one to increase statistical power. Table 2: Resources
Antibodies Sentences Resources Serological assay: We quantitatively measured SARS-CoV-2 IgG antibodies against spike … SciScore for 10.1101/2021.11.29.21267032: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Consent: Written informed consent was obtained from each participant.
IRB: The study design and procedure for data collection were approved by the ethical committee of each center, while those of pooling study were approved by that of the National Center for Global Health and Medicine (NCGM) (the approved number: NCGM-G-004233).Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis In post hoc analysis, we combined the categories of exclusive HNB tobacco users and dual users in one to increase statistical power. Table 2: Resources
Antibodies Sentences Resources Serological assay: We quantitatively measured SARS-CoV-2 IgG antibodies against spike protein in serum using the chemiluminescence enzyme immunoassay (CLEIA) platform (HISCL) manufactured by Sysmex Co. (Kobe, Japan). SARS-CoV-2 IgGsuggested: NoneStatistical analysis: We performed multilevel linear regression analysis to examine the associations of smoking and alcohol use with anti-spike IgG antibody titers in log-scale. anti-spike IgGsuggested: NoneWe drew a restricted cubic spline curve to visualize the dose-response association between alcohol consumption and IgG antibody titers, with 3 knots placed at the 5th, 50th, and 95th percentiles as recommended. IgGsuggested: NoneSoftware and Algorithms Sentences Resources The sensitivity and specificity were reported as 98.3% and 99.6%, respectively.12 We confirmed that IgG antibody titers measured on this assay were highly correlated with those measured on the AdviseDx SARS-CoV-2 IgG II assay (Abbott ARCHITECT®) using data of two centers that measured antibodies with both assays (n=2,961): spearman’s rho = 0.96 (95% confidence intervals [CI]: 0.95–0.96) Abbottsuggested: (Abbott, RRID:SCR_010477)14 Statistical analysis was performed using Stata 17.0 (StataCorp LLC). StataCorpsuggested: (Stata, RRID:SCR_012763)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:We should also acknowledge study limitations. First, as this study is cross-sectional, we cannot determine whether smoking and alcohol use were associated with the surge of antibodies in response to vaccination or the decay of antibodies over time. Second, the number of exclusive HNB tobacco users was not large enough to detect the reduction of IgG titers among them with statistical significance. Third, we did not measure neutralizing antibody, a more reliable marker of immunogenicity. Nevertheless, spike IgG antibody titers measured with the assay we employed are known to well correlate with neutralizing antibody titers (spearman’s ρ=0.71, 95% CI: 0.63–0.77).19 Finally, many East Asians are genetically deficient in one of the enzymes involved in metabolizing alcohol (aldehyde dehydrogenase);11 thus, the present finding regarding alcohol use may not be observed in populations with different genetic background. In conclusion, cigarettes smokers and, to a lesser extent, HNB tobacco product users had lower IgG antibody titers against SAR-CoV-2 spike protein after vaccination, relative to never-smokers. The antibody titers steadily decreased with increasing alcohol consumption, with significant reduction being observed even at moderate amount of alcohol. In addition to conventional cigarette smoking and heavy alcohol use, the use of HNB tobacco products and moderate alcohol drinking may deteriorate immune response to COVID-19 vaccine.
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
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Results from scite Reference Check: We found no unreliable references.
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