A recombinant receptor-binding domain in trimeric form generates protective immunity against SARS-CoV-2 infection in nonhuman primates
This article has been Reviewed by the following groups
Listed in
- Evaluated articles (ScreenIT)
Abstract
No abstract available
Article activity feed
-
-
SciScore for 10.1101/2021.03.30.437647: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable Animals: Five Chinese-origin male rhesus macaques (Macaca mulatta, animal numbers: #140829, #140271, #163957, # 17361, #17321) weighing 4.1 to 8.7 kg and aged 3 to 6 years old were obtained from the Institute of Beijing Xieerxin Biology Resource Co., Ltd. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Subsequently, the plates were incubated with an HRP-conjugated anti-human IgG antibody working solution at 37°C for 30 min and washed with PBST five times. anti-human IgGsuggested: NoneAfter 1 h of incubation, the … SciScore for 10.1101/2021.03.30.437647: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable Animals: Five Chinese-origin male rhesus macaques (Macaca mulatta, animal numbers: #140829, #140271, #163957, # 17361, #17321) weighing 4.1 to 8.7 kg and aged 3 to 6 years old were obtained from the Institute of Beijing Xieerxin Biology Resource Co., Ltd. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Subsequently, the plates were incubated with an HRP-conjugated anti-human IgG antibody working solution at 37°C for 30 min and washed with PBST five times. anti-human IgGsuggested: NoneAfter 1 h of incubation, the membranes were washed five times with TBS containing 0.1% Tween-20, incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (0.1 μg/ml prepared in blocking buffer) as the secondary antibody for one h, and then washed 5 times. anti-rabbit IgGsuggested: NoneThe plates were then incubated with 2 μg/ml biotinylated anti-human IFNγ antibody for 1 h at room temperature, washed six times with sterile PBST, and incubated for 1 h at room temperature with streptavidin–HRP. anti-human IFNγsuggested: NoneAfterward, the cells were washed and surface stained with antibodies against human CD4 (FITC-conjugated), CD8α (FITC-conjugated), and CD44 (FITC-conjugated) for 30 min at room temperature. human CD4suggested: (RayBiotech Cat# CS-11-0133, RRID:AB_1228052)CD8αsuggested: NoneCD44suggested: NoneCells were then washed and incubated with anti-human IFN-γ antibodies (PE-conjugated) for 1 h at room temperature, washed with permeabilization buffer, and fixed in 1% paraformaldehyde. anti-human IFN-γsuggested: NoneThe immunohistochemical reaction was performed on paraffin-embedded lung sections using a rabbit anti-SARS-CoV-2-NP monoclonal antibody followed by an HRP-conjugated goat anti-rabbit secondary antibody as described. anti-SARS-CoV-2-NPsuggested: Noneanti-rabbitsuggested: NoneExperimental Models: Cell Lines Sentences Resources The virus NMDC accession number is NMDCN0000HUI; the isolation strain is 20SF107, which was isolated and titrated in Vero-E6 cells. Vero-E6suggested: NoneThe fused protein was expressed in transfected HEK293 cells and secreted into the cell culture medium. HEK293suggested: CLS Cat# 300192/p777_HEK293, RRID:CVCL_0045)Software and Algorithms Sentences Resources Statistical analysis: All statistical analyses were performed using GraphPad Prism 8.0 software (GraphPad Software, CA, USA). GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
-
-