Neutrophil profiles of pediatric COVID-19 and multisystem inflammatory syndrome in children
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SciScore for 10.1101/2021.12.18.473308: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics IRB: Study Design: Biospecimens were obtained from pediatric patients at Massachusetts General Hospital (MGH) under the institutional review board (IRB) approved ‘MGH Pediatric COVID-19 Biorepository’ (no.
Consent: Informed consent, and assent when appropriate, were obtained in accordance with IRB guidelines from patients and/or parents/guardians before study enrollment.
Field Sample Permit: After plasma collection, PBMCs were isolated and collected via Ficoll density gradient.Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
Software and Algorithms Sentences Resources Differential expression and GSEA results. GSEAsu…SciScore for 10.1101/2021.12.18.473308: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics IRB: Study Design: Biospecimens were obtained from pediatric patients at Massachusetts General Hospital (MGH) under the institutional review board (IRB) approved ‘MGH Pediatric COVID-19 Biorepository’ (no.
Consent: Informed consent, and assent when appropriate, were obtained in accordance with IRB guidelines from patients and/or parents/guardians before study enrollment.
Field Sample Permit: After plasma collection, PBMCs were isolated and collected via Ficoll density gradient.Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
Software and Algorithms Sentences Resources Differential expression and GSEA results. GSEAsuggested: (SeqGSEA, RRID:SCR_005724)Results from OddPub: Thank you for sharing your code.
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:We acknowledge several limitations of our study. First, we utilized bulk transcriptomics due to the technical challenges of performing single-cell RNA-sequencing on neutrophils, so each sample represents a mixture of neutrophils with distinct gene expression programs. However, our high purity double isolation method left little contamination with other cell types. Second, we acknowledge the small size of the cohort, although it represents the largest cohort of its kind due to the low incidence rate of the disease. Third, based on the sample size limitation we were unable to define new gene expression states that may have been specific to pediatric neutrophils and had to rely on the adult-derived signatures, though that work demonstrated a relatively small set of conserved neutrophil states across many different disease contexts. Fourth, MIS-C can present heterogeneously and there was a mixture of treatment regimens and duration of disease among the patients, which may have resulted in greater heterogeneity in the gene expression profiles. All limitations considered, numerous technical challenges and time-sensitive issues were overcome in this study to allow for deep characterization of neutrophil profiles in acute pediatric COVID-19 and MIS-C. In summary, our study provides much needed mechanistic insight into the drivers of pathology in MIS-C and its departure from mild acute SARS-CoV-2 infection in children. We link existing knowledge of gut mucosal breakdown and antigenemi...
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- No funding statement was detected.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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