Longitudinal analysis shows durable and broad immune memory after SARS-CoV-2 infection with persisting antibody responses and memory B and T cells

  1. Kristen W. Cohen
  2. Susanne L. Linderman
  3. Zoe Moodie
  4. Julie Czartoski
  5. Lilin Lai
  6. Grace Mantus
  7. Carson Norwood
  8. Lindsay E. Nyhoff
  9. Venkata Viswanadh Edara
  10. Katharine Floyd
  11. Stephen C. De Rosa
  12. Hasan Ahmed
  13. Rachael Whaley
  14. Shivan N. Patel
  15. Brittany Prigmore
  16. Maria P. Lemos
  17. Carl W. Davis
  18. Sarah Furth
  19. James B. O’Keefe
  20. Mohini P. Gharpure
  21. Sivaram Gunisetty
  22. Kathy Stephens
  23. Rustom Antia
  24. Veronika I. Zarnitsyna
  25. David S. Stephens
  26. Srilatha Edupuganti
  27. Nadine Rouphael
  28. Evan J. Anderson
  29. Aneesh K. Mehta
  30. Jens Wrammert
  31. Mehul S. Suthar
  32. Rafi Ahmed
  33. M. Juliana McElrath

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Abstract

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  1. Version published to 10.1016/j.xcrm.2021.100354
  2. Version published to 10.1101/2021.04.19.21255739v2 on medRxiv
  3. ScreenIT

    SciScore for 10.1101/2021.04.19.21255739: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    EthicsIRB: Study Populations: Two longitudinal COVID-19 cohort studies at Fred Hutchinson Cancer Research Center (Seattle, Washington) and Emory University (Atlanta, Georgia) began after receiving institutional review board approvals (IRB 10440, IRB 00001080 and IRB00022371).
    Consent: Adults ≥18 years were enrolled who met eligibility criteria for SARS-CoV-2 infection and provided informed consent.
    Sex as a biological variablenot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    Participants undiagnosed with COVID-19 had a nasopharyngeal (NP) swab collected and tested for SARS-CoV-2 via an FDA-approved PCR test and blood was collected for SARS-CoV-2 antibody (Abbott) and study assays.
    SARS-CoV-2
    suggested: None
    Following a wash, plates were incubated with 50ul/well of Sulfo-Tag anti-human IgG, IgA, or IgM detection antibody and shaken at 700RPM at room temperature for 1 hr.
    anti-human IgG
    suggested: None
    The antibody-virus mixture was added to VeroE6 cell (C1008, ATCC,
    C1008
    suggested: None
    Experimental Models: Cell Lines
    SentencesResources
    The antibody-virus mixture was added to VeroE6 cell (C1008, ATCC,
    VeroE6
    suggested: JCRB Cat# JCRB1819, RRID:CVCL_YQ49)
    Experimental Models: Organisms/Strains
    SentencesResources
    Study Populations: Two longitudinal COVID-19 cohort studies at Fred Hutchinson Cancer Research Center (Seattle, Washington) and Emory University (Atlanta, Georgia) began after receiving institutional review board approvals (IRB 10440, IRB 00001080 and IRB00022371).
    IRB 00001080
    suggested: None
    Software and Algorithms
    SentencesResources
    Participants undiagnosed with COVID-19 had a nasopharyngeal (NP) swab collected and tested for SARS-CoV-2 via an FDA-approved PCR test and blood was collected for SARS-CoV-2 antibody (Abbott) and study assays.
    Abbott
    suggested: (Abbott, RRID:SCR_010477)
    The FRNT-mNG50 titers were interpolated using a 4-parameter nonlinear regression in GraphPad Prism 8.4.3.
    GraphPad Prism
    suggested: (GraphPad Prism, RRID:SCR_002798)
    Data was analyzed in Flow Jo version 9.9.4
    Flow Jo
    suggested: (FlowJo, RRID:SCR_008520)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    Results from scite Reference Check: We found no unreliable references.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  4. Version published to 10.1101/2021.04.19.21255739v1 on medRxiv