Intrinsic signal amplification by type III CRISPR-Cas systems provides a sequence-specific SARS-CoV-2 diagnostic

Article activity feed

1. Version published to 10.1016/j.xcrm.2021.100319

   Jun 1, 2021
2. 

   ScreenIT

   Mar 1, 2021

   SciScore for 10.1101/2020.10.14.20212670: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.

   Table 2: Resources

   Software and Algorithms
   Sentences	Resources
   The T7-FIP primer consists of a T7 promoter fused to the 3’ half of the FIP primer, and allows for the generation of T7 transcription templates during LAMP (Supplemental Table 1).	LAMP suggested: (LAMP, RRID:SCR_001740)
   Bioinformatic analyses: 45,652 SARS-CoV-2 genomes defined as complete (>29,000 bp) were downloaded from GISAID (https://www.gisaid.org/) (Elbe and Buckland-Merrett, 2017), on June 23, 2020.	Bioinformatic suggested: (QFAB Bioinformatics, RRID:SCR_012513)
   The …

   SciScore for 10.1101/2020.10.14.20212670: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.

   Table 2: Resources

   Software and Algorithms
   Sentences	Resources
   The T7-FIP primer consists of a T7 promoter fused to the 3’ half of the FIP primer, and allows for the generation of T7 transcription templates during LAMP (Supplemental Table 1).	LAMP suggested: (LAMP, RRID:SCR_001740)
   Bioinformatic analyses: 45,652 SARS-CoV-2 genomes defined as complete (>29,000 bp) were downloaded from GISAID (https://www.gisaid.org/) (Elbe and Buckland-Merrett, 2017), on June 23, 2020.	Bioinformatic suggested: (QFAB Bioinformatics, RRID:SCR_012513)
   The generation and viewing of viral genome alignments was performed using publicly available software, including SnapGene Viewer and RStudio V1.2.5001 (Methods).	SnapGene suggested: (SnapGene, RRID:SCR_015052)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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3. 

   Version published to 10.1101/2020.10.14.20212670 on medRxiv

   Oct 20, 2020