SARS-CoV-2 protein subunit vaccination of mice and rhesus macaques elicits potent and durable neutralizing antibody responses

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1. Version published to 10.1016/j.xcrm.2021.100252

   Apr 1, 2021
2. 

   ScreenIT

   Mar 1, 2021

   SciScore for 10.1101/2020.07.31.228486: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IACUC: Ethics statement: The animal work was conducted with the approval of the regional Ethical Committee on Animal Experiments (Stockholms Norra Djurförsök-setiska Nämnd).
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	Whole blood was allowed to clot at room temperature, and serum was separated by centrifugation, heat inactivated at 56°C for 1 hour, and stored at -20°C until use. Macaques: Two female and one male rhesus macaques (Macaca mulatta) of Chinese origin, 4-5 years old, were housed at the Astrid Fagraeus Laboratory at Karolinska Institutet.
   Cell Line Authentication	Contamination: All cell lines …

   SciScore for 10.1101/2020.07.31.228486: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IACUC: Ethics statement: The animal work was conducted with the approval of the regional Ethical Committee on Animal Experiments (Stockholms Norra Djurförsök-setiska Nämnd).
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	Whole blood was allowed to clot at room temperature, and serum was separated by centrifugation, heat inactivated at 56°C for 1 hour, and stored at -20°C until use. Macaques: Two female and one male rhesus macaques (Macaca mulatta) of Chinese origin, 4-5 years old, were housed at the Astrid Fagraeus Laboratory at Karolinska Institutet.
   Cell Line Authentication	Contamination: All cell lines tested negative for mycoplasma by PCR.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Plates were washed 6 times with PBS-T, and 100µl of a goat anti-Mouse IgG horseradish peroxidase (HRP) conjugated secondary antibody (Southern Biotech) diluted 1:5,000 in PBS-T was added to each well.	anti-Mouse IgG suggested: None
   Plates were washed and antibody-antigen interaction was detected using HRP-conjugated anti-monkey IgG Fc (#GAMon/IgG(Fc)/PO Nordic MUbio) diluted 1:20,000 in PBS-T.	anti-monkey IgG Fc suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   Pseudotyped neutralization assays: Pseudotyped neutralization assays were adapted from protocols previously validated to characterize the neutralization of HIV 42 but with the use of HEK293T-ACE2 cells, as previously described 17.	HEK293T-ACE2 suggested: None
   Pseudotyped lentiviruses displaying the SARS-CoV-2 spike protein (harboring an 18 amino acid truncation of the cytoplasmic tail) and packaging a luciferase reporter gene were generated by the co-transfection of HEK293T cells using Lipofectamine 3000 (In-vitrogen) per the manufacturer’s protocols.	HEK293T suggested: None
   Experimental Models: Organisms/Strains
   Sentences	Resources
   Mice: C57BL/6J (Jackson Laboratory) wild-type mice were housed and bred at Karolinska Institutet animal facility.	C57BL/6J suggested: RRID:IMSR_JAX:000664)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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3. 

   Version published to 10.1101/2020.07.31.228486v1 on bioRxiv

   Jul 31, 2020