Cross-Sectional Evaluation of Humoral Responses against SARS-CoV-2 Spike
This article has been Reviewed by the following groups
Listed in
- Evaluated articles (ScreenIT)
Abstract
No abstract available
Article activity feed
-
-
-
SciScore for 10.1101/2020.06.08.140244: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Consent: Ethics statement: All work was conducted in accordance with the Declaration of Helsinki in terms of informed consent and approval by an appropriate institutional board. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Alexa Fluor-647-conjugated goat anti-human IgG (H+L) Abs (Invitrogen) were used as secondary antibodies to detect sera binding in flow cytometry experiment. anti-human IgGsuggested: NoneExperimental Models: Cell Lines Sentences Resources 293T-ACE2 cells were then cultured in a medium supplemented … SciScore for 10.1101/2020.06.08.140244: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Consent: Ethics statement: All work was conducted in accordance with the Declaration of Helsinki in terms of informed consent and approval by an appropriate institutional board. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Alexa Fluor-647-conjugated goat anti-human IgG (H+L) Abs (Invitrogen) were used as secondary antibodies to detect sera binding in flow cytometry experiment. anti-human IgGsuggested: NoneExperimental Models: Cell Lines Sentences Resources 293T-ACE2 cells were then cultured in a medium supplemented with 2 μg/ml of puromycin (Sigma). 293T-ACE2suggested: RRID:CVCL_YZ65)Flow cytometry analysis of cell-surface staining: Using the standard calcium phosphate method, 10μg of Spike expressor and 2μg of a green fluorescent protein (GFP) expressor (pIRES-GFP) was transfected into 2 × 106 293T cells. 293Tsuggested: NCBI_Iran Cat# C498, RRID:CVCL_0063)Software and Algorithms Sentences Resources Samples were acquired on a LSRII cytometer (BD Biosciences, Mississauga, ON, Canada) and data analysis was performed using FlowJo vX. FlowJosuggested: (FlowJo, RRID:SCR_008520)Dendrograms were calculated using the dendPlot function and hclust method, or as implemented in the heatmap package in R. hclustsuggested: (HCLUST, RRID:SCR_009154)Chord diagrams were generated in R and R Studio based on the circlize and ComplexHeatmap package, as recently described. circlizesuggested: (circlize, RRID:SCR_002141)ComplexHeatmapsuggested: (ComplexHeatmap, RRID:SCR_017270)Forrest plots and calculations of fold change, significance (Mann-Whitney) and adjusted P values (Holm-Sidak) were done using Excel and Prism v8.2.0. Excelsuggested: NonePrismsuggested: (PRISM, RRID:SCR_005375)The confidence interval of a quotient of two means was calculated based on the Fieller method using GraphPad QuickCalcs. GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)Statistical analyses: Statistics were analyzed using GraphPad Prism version 8.0.2 (GraphPad, San Diego, CA, (USA). GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
-
-