Rapid Generation of Neutralizing Antibody Responses in COVID-19 Patients

  1. Mehul S. Suthar
  2. Matthew G. Zimmerman
  3. Robert C. Kauffman
  4. Grace Mantus
  5. Susanne L. Linderman
  6. William H. Hudson
  7. Abigail Vanderheiden
  8. Lindsay Nyhoff
  9. Carl W. Davis
  10. Oluwaseyi Adekunle
  11. Maurizio Affer
  12. Melanie Sherman
  13. Stacian Reynolds
  14. Hans P. Verkerke
  15. David N. Alter
  16. Jeannette Guarner
  17. Janetta Bryksin
  18. Michael C. Horwath
  19. Connie M. Arthur
  20. Natia Saakadze
  21. Geoffrey H. Smith
  22. Srilatha Edupuganti
  23. Erin M. Scherer
  24. Kieffer Hellmeister
  25. Andrew Cheng
  26. Juliet A. Morales
  27. Andrew S. Neish
  28. Sean R. Stowell
  29. Filipp Frank
  30. Eric Ortlund
  31. Evan J. Anderson
  32. Vineet D. Menachery
  33. Nadine Rouphael
  34. Aneesh K. Mehta
  35. David S. Stephens
  36. Rafi Ahmed
  37. John D. Roback
  38. Jens Wrammert

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Abstract

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  1. ScreenIT

    SciScore for 10.1101/2020.05.03.20084442: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board StatementIRB: All collection, processing, and archiving of human specimens was performed under approval from the University Institutional Review Board (IRB #00000510 and #00022371).
    Consent: For IRB #00000510, informed consent was obtained prior to patient participation.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    Preparation of CR3022 monoclonal antibody and biotinylation.
    CR3022
    suggested: None
    Biotinylated versions of CR3022 used in viral neutralization assays were produced by combining the antibody with a 20 molar excess of EZ-Link NHS-PEG4-Biotin (Thermo Fisher Scientific) for 1 hour at room temperatures.
    NHS-PEG4-Biotin
    suggested: None
    Experimental Models: Cell Lines
    SentencesResources
    Vero cells were cultured in complete DMEM medium consisting of 1x DMEM (Corning Cellgro), 10% FBS, 25mM HEPES Buffer (Corning Cellgro), 2mM L-glutamine, 1mM sodium pyruvate, 1x Non-essential Amino Acids, and 1x antibiotics.
    Vero
    suggested: CLS Cat# 605372/p622_VERO, RRID:CVCL_0059)

    Results from OddPub: Thank you for sharing your data.

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  2. Version published to 10.1016/j.xcrm.2020.100040
  3. Version published to 10.1101/2020.05.03.20084442 on medRxiv