Biomarkers selection for population normalization in SARS-CoV-2 wastewater-based epidemiology

Article activity feed

1. Version published to 10.1016/j.watres.2022.118985

   Sep 1, 2022
2. 

   ScreenIT

   Mar 17, 2022

   SciScore for 10.1101/2022.03.14.22272359: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   NIH rigor criteria are not applicable to paper type.

   Table 2: Resources

   Recombinant DNA
   Sentences	Resources
   The data would be collected at the step of 55°C extension. 2.4 Quantification of biomarkers: 2.4.1 Detection of pMMoV viral concentration: The TaqMan probe (pMMoV Probe) and the primer pair (pMMoV Forward and pMMoV Reverse, Table 2) were designed and used to target the pMMoV RNA.	pMMoV suggested: None
   Software and Algorithms
   Sentences	Resources
   2.3 Detection of SARS-CoV-2 concentration: 2.3.1 RNA extraction from wastewater samples: Fifty mL of wastewater from each catchment was filtered through a 0.22-micron filter (Millipore cat# SCGPOO525).	SARS-CoV-2 suggested: (Active Motif Cat# 91351, RRID:AB_28478…

   SciScore for 10.1101/2022.03.14.22272359: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   NIH rigor criteria are not applicable to paper type.

   Table 2: Resources

   Recombinant DNA
   Sentences	Resources
   The data would be collected at the step of 55°C extension. 2.4 Quantification of biomarkers: 2.4.1 Detection of pMMoV viral concentration: The TaqMan probe (pMMoV Probe) and the primer pair (pMMoV Forward and pMMoV Reverse, Table 2) were designed and used to target the pMMoV RNA.	pMMoV suggested: None
   Software and Algorithms
   Sentences	Resources
   2.3 Detection of SARS-CoV-2 concentration: 2.3.1 RNA extraction from wastewater samples: Fifty mL of wastewater from each catchment was filtered through a 0.22-micron filter (Millipore cat# SCGPOO525).	SARS-CoV-2 suggested: (Active Motif Cat# 91351, RRID:AB_2847848)
   The specificity of primers and probe were tested by BLAST analysis (NCBI) to prevent known nonspecific binding targets that could be obtained in a human specimen.	BLAST suggested: (BLASTX, RRID:SCR_001653)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Results from scite Reference Check: We found no unreliable references.

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3. 

   Version published to 10.1101/2022.03.14.22272359v1 on medRxiv

   Mar 15, 2022