Identification and quantification of bioactive compounds suppressing SARS-CoV-2 signals in wastewater-based epidemiology surveillance
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SciScore for 10.1101/2022.03.09.22272155: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
Software and Algorithms Sentences Resources Metabolites of significant features (p < 0.001 and intensity ≥10,000) were putatively identified by the integration of the METLIN database with XCMS Online. METLINsuggested: (METLIN, RRID:SCR_010500)To further characterize and visualize the differences in profiles of compounds among different facilities, partial least squares-discriminant analysis (PLS-DA) was performed and heatmap was generated via the web-based tool MetaboAnalyst (Wishart Research Group, University of Alberta, Alberta, Canada) [47]. 2.8 Met…SciScore for 10.1101/2022.03.09.22272155: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
Software and Algorithms Sentences Resources Metabolites of significant features (p < 0.001 and intensity ≥10,000) were putatively identified by the integration of the METLIN database with XCMS Online. METLINsuggested: (METLIN, RRID:SCR_010500)To further characterize and visualize the differences in profiles of compounds among different facilities, partial least squares-discriminant analysis (PLS-DA) was performed and heatmap was generated via the web-based tool MetaboAnalyst (Wishart Research Group, University of Alberta, Alberta, Canada) [47]. 2.8 MetaboAnalystsuggested: (MetaboAnalyst, RRID:SCR_015539)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:Due to the limitations of the instrument and limited availability of chemical references standards, fourteen compounds out of thirty were detected and quantified (Table 4) and (Table 5). Table 4 summarizes the molecular ions, product ions, retention times, and ionization modes for targeted LC-MSMS analysis of these compounds. The results showed that most of the bioactive compounds had higher concentrations in the wastewater of facilities exhibiting SARS-CoV-2 signal suppression than the control facilities. Four compounds had much higher concentrations in the suppression facilities than the control facilities. In particular, 4-nonylphenol, palmitelaidic acid, sodium oleate, and polyethyleneglycol dioleate exhibited concentrations that were 73.3%, 35.3%, 54%, and 58.8% higher in the suppression facilities than the control facilities, respectively (Figure 6). These compounds are mainly used in the production of surfactants and detergents in various industries [53, 54] The concentrations of 4-nonylphenol in the urban wastewaters were determined in Japan, China, and USA. The concentrations were about 190 µg/L [55], 2 µg/L [56], and 400 µg/L [57], respectively. In this study, average concentrations of 4-nonylphenol were 1169 ± 13.3 µg/L and 2025.7 ± 247 µg/L in the control and suppression facilities, respectively. No information was found regarding the concentrations of the other three compounds in wastewater. Palmitelaidic acid was reported to be used to produce cosmetics, soaps, ...
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
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- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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