Longitudinal SARS-CoV-2 RNA wastewater monitoring across a range of scales correlates with total and regional COVID-19 burden in a well-defined urban population
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SciScore for 10.1101/2021.11.19.21266588: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Field Sample Permit: Sample collection from WWTP-2, WWTP-3 and neighborhood locations were time-weighted, whereas for samples collected from the larger WWTP-1 were flow-weighted.
IACUC: This study received institutional ethics approval from the Conjoint Regional Health Ethics Board of the University of Calgary (REB20-1544). 2.4.
IRB: This study received institutional ethics approval from the Conjoint Regional Health Ethics Board of the University of Calgary (REB20-1544). 2.4.Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication Authentication: For each day, total-active cases (i.e., confirmed by validated … SciScore for 10.1101/2021.11.19.21266588: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Field Sample Permit: Sample collection from WWTP-2, WWTP-3 and neighborhood locations were time-weighted, whereas for samples collected from the larger WWTP-1 were flow-weighted.
IACUC: This study received institutional ethics approval from the Conjoint Regional Health Ethics Board of the University of Calgary (REB20-1544). 2.4.
IRB: This study received institutional ethics approval from the Conjoint Regional Health Ethics Board of the University of Calgary (REB20-1544). 2.4.Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication Authentication: For each day, total-active cases (i.e., confirmed by validated clinical RT-qPCR assays) and newly diagnosed cases were collected by Alberta Health Services (AHS) (https://covid-tracker.chi-csm.ca/) and binned by individual postal codes (using the first three of six digits) as indicated by the home address of newly diagnosed cases. Table 2: Resources
No key resources detected.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:There are limitations to assigning clinical case residence based on three-digit postal codes, as these boundaries do not necessarily align precisely with sewershed drainage networks. Sampling at larger WWTP and city-wide scales result in these potential ‘overlap artifacts’ fading away, resulting in stronger correlations for these larger catchments and populations. This limitation can in principle be accounted for in future efforts where granular WBE data is sought. Novel strategies that maintain individuals’ anonymity while facilitating precise attribution of diagnosed cases to addresses can be developed with the guidance of local ethics boards (Hrudey et al., 2021). Other factors may also contribute to improved correlation of SARS-CoV-2 wastewater signals with clinically identified cases at larger geographies. In areas of lower SES and higher occupancy per residence (i.e., crowding), secondary infection rates within a household are higher potentially concentrating the wastewater signal (Koureas et al., 2021; Madewell et al., 2021). This is consistent with evidence that the adherence to physical and social distancing recommendations are correlated with SES indicators (Jay et al., 2020). A major limitation of wastewater surveillance is that the signal recorded is only contributed to by individuals who defecate in a particular sewershed catchment. Indeed, most studies either show no or extremely low levels of SARS-CoV-2 excreted in urine (with ≥10 more RNA shed/g in stool relat...
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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