A multicenter study investigating SARS-CoV-2 in tertiary-care hospital wastewater. viral burden correlates with increasing hospitalized cases as well as hospital-associated transmissions and outbreaks
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SciScore for 10.1101/2021.02.20.21251520: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: This research was approved by the University of Calgary’s Conjoint Health Regional Ethics Board ( Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
No key resources detected.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:A key limitation to the identification of SARS-CoV-2 in wastewater samples, relative to clinical samples (e.g., swabs) is the massive …
SciScore for 10.1101/2021.02.20.21251520: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: This research was approved by the University of Calgary’s Conjoint Health Regional Ethics Board ( Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
No key resources detected.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:A key limitation to the identification of SARS-CoV-2 in wastewater samples, relative to clinical samples (e.g., swabs) is the massive volume of water in which samples are diluted. This necessitates sample concentration. While procedures for the efficient recovery of non-enveloped viruses exist, researchers continue to search for satisfactory protocols for enveloped viruses such as SARS-CoV-2[27]. Many groups have explored ways to improve the sensitivity of wastewater SARS-CoV-2 detection. Diagnostic platforms with improved sensitivity and more impervious to impurities in the wastewater matrix (i.e., digital droplet RT-PCR) also show considerable promise[28, 29]. Sampling in the proximal sewershed may lead to day-to-day variance resulting in signal noise. This is particularly true for single-facility studies where potential extremes in individual virus shedding could confound results; limited data suggests significant variations in fecal viral load occur (from 103.4 to 107.6)[30]. This is a key challenge that remains to be solved if wastewater data is to be used in a meaningful way. Similarly, issues arise with attempts to normalize SARS-CoV-2 based on the contributing population. We chose to use the PMMoV as a fecal biomarker to control for variations in fecal loading – a particular risk when sampling takes place ‘upstream’ in the sewershed. While this marker has been validated in WW-TP samples where large and diverse populations contribute to sewage[31], hospitals are a much...
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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