Ultra-fast and onsite interrogation of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in waters via surface enhanced Raman scattering (SERS)
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SciScore for 10.1101/2020.05.02.20086876: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization ., LTD.) and formed randomly on a 4-inch silicon wafer with increasing deposition time35. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources The GLAD stage was then tilt to 84° with respect to the incident vapor. GLADsuggested: (GLAD, RRID:SCR_001284)2.5 Multivariate analysis of Raman spectra: Raw spectral data were pre-processed by using the open source IRootLab toolbox performed on MATLAB r2012 36 Briefly, each acquired Raman spectrum was cut to a biochemical-cell fingerprint region (900-1800 cm−1), baseline corrected, wavelet de-noised, … SciScore for 10.1101/2020.05.02.20086876: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization ., LTD.) and formed randomly on a 4-inch silicon wafer with increasing deposition time35. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources The GLAD stage was then tilt to 84° with respect to the incident vapor. GLADsuggested: (GLAD, RRID:SCR_001284)2.5 Multivariate analysis of Raman spectra: Raw spectral data were pre-processed by using the open source IRootLab toolbox performed on MATLAB r2012 36 Briefly, each acquired Raman spectrum was cut to a biochemical-cell fingerprint region (900-1800 cm−1), baseline corrected, wavelet de-noised, and vector normalized. MATLABsuggested: (MATLAB, RRID:SCR_001622)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:There are some limitations in this work. Firstly, our ACE2@SN-SERS assay cannot evaluate viral viability or infectivity as free spike proteins or viral envelop are also recognizable by ACE2@SN-SERS substrates to quench SERS signals, possibly overestimating the presence of SARS-CoV-2 in human or environmental specimens. Secondly, only limited real samples were tested and the interference of other viruses targeting ACE2 for cell entry is still questionable, owing to the restriction in sample collection and clinical tests during the outbreak of COVID-19 in Wuhan. Nevertheless, the mentioned advantages prove ACE2@SN-SERS assay as a reliable and for mobile detection platform or screening system for clinal and environmental diagnosis onsite under a variety of conditions. Our state-of-the-art work also raises a concept to screen other viruses with RBD recognizing receptors of human cells for entry and evaluate the recognition strength of SARS-like viruses across mammalian species, when different RBD-containing viruses are tested or human cell receptors are functionalized for substrate fabrication. As a possible in-vitro assay, ACE2@SN-SERS substrate might also contribute to the assessment of vaccine efficiency. Further studies need to address those possibilities and establish robust databases and algorithms for a faster interrogation, even down to 1 min, for clinical and environmental purposes.
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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