SARS-CoV-2 RNA in wastewater anticipated COVID-19 occurrence in a low prevalence area
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SciScore for 10.1101/2020.04.22.20075200: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
No key resources detected.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:Nonetheless, the need of a BSL3 laboratory facility to handle SARS-CoV-2 represents the main limitation of this experiment. 3.2. SARS-CoV-2 titers in wastewater and effluent water: A total of 42 influent, and 18 secondary and …
SciScore for 10.1101/2020.04.22.20075200: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
No key resources detected.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:Nonetheless, the need of a BSL3 laboratory facility to handle SARS-CoV-2 represents the main limitation of this experiment. 3.2. SARS-CoV-2 titers in wastewater and effluent water: A total of 42 influent, and 18 secondary and 12 tertiary treated effluent water samples were collected from 12 March to 14 April 2020 and investigated for the occurrence of SARS-CoV-2 RNA. Samples were considered positive for Ct below 40 (as in Medema et al., 2020 and F. Wu et al., 2020) and titrated by using the quantified plasmid control for each of the RT-qPCR targets. The 83.3% (35 positive samples out of 42) influent samples and the 11.1% (2 out of 18) secondary treated water samples were tested positive for at least one SARS-CoV-2 RT-qPCR target. None of the tertiary effluent samples (0 out of 12) tested positive for any of the SARS-CoV-2 RT-qPCR target (Figure 2). A relevant number of influent water samples (12%) showed Ct ranging between 37 and 40, even though lower Ct of 34-37 were observed (29%). In all samples, MgV recoveries were above 1% (10.05 ± 14.10%). On average, SARS-CoV-2 RNA titers of 5.15 ± 0.25, 5.53 ± 0.24, and 5.49 ± 0.27 log gc/L were quantified in wastewater by using N1, N2 and N3 primer/probe mixes, respectively. Titers of 4 and 5 to more than 6 log gc/L have been reported in Massachusetts and France, respectively (F. Wu et al., 2020; Wurtzer et al., 2020). A secondary effluent sample resulted positive for N2 and quantified as 5.40 log gc/L. An additional secondary efflue...
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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