Increased histone-DNA complexes and endothelial-dependent thrombin generation in severe COVID-19
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SciScore for 10.1101/2021.07.03.450992: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Consent: All participants provided written consent using a protocol approved by National Jewish Health IRB.
IRB: Healthy donors (n=6) were recruited and provided written consented using a protocol approved by the University of Vermont Committee on Human Research.Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Plasma was incubated with the thrombin substrate Z-Gly-Gly-Arg 7-amido-4-methylcoumarin hydrochloride (0.42 mM) (Bachem AG, Switzerland) and CaCl2 (15 mM) (3 min, 37°C), and the reactions initiated by the addition of … SciScore for 10.1101/2021.07.03.450992: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Consent: All participants provided written consent using a protocol approved by National Jewish Health IRB.
IRB: Healthy donors (n=6) were recruited and provided written consented using a protocol approved by the University of Vermont Committee on Human Research.Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Plasma was incubated with the thrombin substrate Z-Gly-Gly-Arg 7-amido-4-methylcoumarin hydrochloride (0.42 mM) (Bachem AG, Switzerland) and CaCl2 (15 mM) (3 min, 37°C), and the reactions initiated by the addition of relipidated tissue factor1-242 (6.5 pM) (a gift from Dr. R. Lunblad, Baxter Healthcare Corp.) and synthetic vesicles consisting of 80% phosphatidylcholine and 20% phosphatidylserine (PCPS) (20 µM), or EA.hy926 cells (2×105). EA.hy926suggested: BCRJ Cat# 0345, RRID:CVCL_3901)Software and Algorithms Sentences Resources Plasma was incubated with the thrombin substrate Z-Gly-Gly-Arg 7-amido-4-methylcoumarin hydrochloride (0.42 mM) (Bachem AG, Switzerland) and CaCl2 (15 mM) (3 min, 37°C), and the reactions initiated by the addition of relipidated tissue factor1-242 (6.5 pM) (a gift from Dr. R. Lunblad, Baxter Healthcare Corp.) and synthetic vesicles consisting of 80% phosphatidylcholine and 20% phosphatidylserine (PCPS) (20 µM), or EA.hy926 cells (2×105). Baxter Healthcaresuggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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