SARS-CoV-2 spike protein-mediated cell signaling in lung vascular cells

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1. Version published to 10.1016/j.vph.2020.106823

   Apr 1, 2021
2. 

   ScreenIT

   Oct 15, 2020

   SciScore for 10.1101/2020.10.12.335083: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IACUC: Clinical studies were approved by the regional committee for medical research ethics in Kiev, Ukraine and performed under the Helsinki Declaration of 1975 revised in 2013 or comparable ethical standards.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Some cells were pretreated for 1 h with the rabbit anti-ACE2 antibody (Catalog # 4355; Cell Signaling Technology, Danvers, MA).	anti-ACE2 suggested: (Cell Signaling Technology Cat# 4355, RRID:AB_2797606)
   The membrane was blocked with Odyssey blocking buffer (LI-COR, Lincoln, NE, USA) for one hour at …

   SciScore for 10.1101/2020.10.12.335083: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IACUC: Clinical studies were approved by the regional committee for medical research ethics in Kiev, Ukraine and performed under the Helsinki Declaration of 1975 revised in 2013 or comparable ethical standards.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Some cells were pretreated for 1 h with the rabbit anti-ACE2 antibody (Catalog # 4355; Cell Signaling Technology, Danvers, MA).	anti-ACE2 suggested: (Cell Signaling Technology Cat# 4355, RRID:AB_2797606)
   The membrane was blocked with Odyssey blocking buffer (LI-COR, Lincoln, NE, USA) for one hour at 25°C and incubated overnight with the rabbit anti-phospho-MEK1/2 (Ser217/221) antibody (Catalog #9154; Cell Signaling), the mouse anti-MEK1/2 antibody (Catalog #4694; Cell Signaling), the rabbit anti-phospho-Akt (Ser473) antibody (Catalog #9271; Cell Signaling), or the rabbit anti-phospho-Stat3 (Tyr705) antibody (Catalog #9131; Cell Signaling) at 4°C.	anti-phospho-MEK1/2 suggested: (Cell Signaling Technology Cat# 9154, RRID:AB_2138017) Ser217/221 suggested: (Cell Signaling Technology Cat# 9154, RRID:AB_2138017) anti-MEK1/2 suggested: (Cell Signaling Technology Cat# 9122, RRID:AB_823567) anti-phospho-Akt suggested: (Cell Signaling Technology Cat# 9271, RRID:AB_329825) Ser473 suggested: (Cell Signaling Technology Cat# 9271, RRID:AB_329825) anti-phospho-Stat3 (Tyr705) suggested: (Cell Signaling Technology Cat# 9138, RRID:AB_331262)
   Software and Algorithms
   Sentences	Resources
   Morphometric investigations included the assessment of pulmonary arterial wall thickness and pulmonary arterial lumen index (the ratio of internal vessel area to external vessel area) using the ImageJ software.	ImageJ suggested: (ImageJ, RRID:SCR_003070)
   For the morphometric analysis, IBM SPSS Statistics software version 22.0 was used for the statistical calculations.	SPSS suggested: (SPSS, RRID:SCR_002865)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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3. 

   Version published to 10.1101/2020.10.12.335083v1 on bioRxiv

   Oct 12, 2020