SARS-CoV-2 spike protein-mediated cell signaling in lung vascular cells
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SciScore for 10.1101/2020.10.12.335083: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IACUC: Clinical studies were approved by the regional committee for medical research ethics in Kiev, Ukraine and performed under the Helsinki Declaration of 1975 revised in 2013 or comparable ethical standards. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Antibodies Sentences Resources Some cells were pretreated for 1 h with the rabbit anti-ACE2 antibody (Catalog # 4355; Cell Signaling Technology, Danvers, MA). anti-ACE2suggested: (Cell Signaling Technology Cat# 4355, RRID:AB_2797606)The membrane was blocked with Odyssey blocking buffer (LI-COR, Lincoln, NE, USA) for one hour at … SciScore for 10.1101/2020.10.12.335083: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IACUC: Clinical studies were approved by the regional committee for medical research ethics in Kiev, Ukraine and performed under the Helsinki Declaration of 1975 revised in 2013 or comparable ethical standards. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Antibodies Sentences Resources Some cells were pretreated for 1 h with the rabbit anti-ACE2 antibody (Catalog # 4355; Cell Signaling Technology, Danvers, MA). anti-ACE2suggested: (Cell Signaling Technology Cat# 4355, RRID:AB_2797606)The membrane was blocked with Odyssey blocking buffer (LI-COR, Lincoln, NE, USA) for one hour at 25°C and incubated overnight with the rabbit anti-phospho-MEK1/2 (Ser217/221) antibody (Catalog #9154; Cell Signaling), the mouse anti-MEK1/2 antibody (Catalog #4694; Cell Signaling), the rabbit anti-phospho-Akt (Ser473) antibody (Catalog #9271; Cell Signaling), or the rabbit anti-phospho-Stat3 (Tyr705) antibody (Catalog #9131; Cell Signaling) at 4°C. anti-phospho-MEK1/2suggested: (Cell Signaling Technology Cat# 9154, RRID:AB_2138017)Ser217/221suggested: (Cell Signaling Technology Cat# 9154, RRID:AB_2138017)anti-MEK1/2suggested: (Cell Signaling Technology Cat# 9122, RRID:AB_823567)anti-phospho-Aktsuggested: (Cell Signaling Technology Cat# 9271, RRID:AB_329825)Ser473suggested: (Cell Signaling Technology Cat# 9271, RRID:AB_329825)anti-phospho-Stat3 (Tyr705)suggested: (Cell Signaling Technology Cat# 9138, RRID:AB_331262)Software and Algorithms Sentences Resources Morphometric investigations included the assessment of pulmonary arterial wall thickness and pulmonary arterial lumen index (the ratio of internal vessel area to external vessel area) using the ImageJ software. ImageJsuggested: (ImageJ, RRID:SCR_003070)For the morphometric analysis, IBM SPSS Statistics software version 22.0 was used for the statistical calculations. SPSSsuggested: (SPSS, RRID:SCR_002865)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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