Reduced levels of convalescent neutralizing antibodies against SARS-CoV-2 B.1+L249S+E484K lineage

  1. Diego A. Álvarez-Díaz
  2. Katherine Laiton-Donato
  3. Orlando Alfredo Torres-García
  4. Hector Alejandro Ruiz-Moreno
  5. Carlos Franco-Muñoz
  6. Maria Angie Beltran
  7. Marcela Mercado-Reyes
  8. Miguel Germán Rueda
  9. Ana Luisa Muñoz

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Abstract

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  1. Version published to 10.1016/j.virusres.2021.198629
  2. ScreenIT

    SciScore for 10.1101/2021.09.13.21263430: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    EthicsConsent: 2.1 Human subject collection: The samples were collected between March 2020 and February 2021; all subjects enrolled in this research responded voluntarily to an informed consent formulary previously approved by the Ethics Committee of Colombian National Health Institute
    IRB: 2.1 Human subject collection: The samples were collected between March 2020 and February 2021; all subjects enrolled in this research responded voluntarily to an informed consent formulary previously approved by the Ethics Committee of Colombian National Health Institute
    Sex as a biological variablenot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    Additionally, SARS-CoV2 IgG anti-Nucleocapsid antibodies detection on the Allinity ci series (Abbott, Chicago,IL, USA) was done as screening, where relative light units (RLU) >1.4 were interpreted as positive.
    anti-Nucleocapsid
    suggested: None
    Experimental Models: Cell Lines
    SentencesResources
    This study was conducted in compliance with ethical principles of the Declaration of Helsinki and to the conditions provided by the Ministry of Health - Colombia. 2.2 Cells: African green monkey kidney Vero E6 cells (ATCC CRL-1586™) were used to propagate the SARS-CoV-2 isolates and the neutralization assays.
    Vero E6
    suggested: None
    Software and Algorithms
    SentencesResources
    The sequence dataset was aligned using the MAFFT software v7 (26,27).
    MAFFT
    suggested: (MAFFT, RRID:SCR_011811)
    Additionally, SARS-CoV2 IgG anti-Nucleocapsid antibodies detection on the Allinity ci series (Abbott, Chicago,IL, USA) was done as screening, where relative light units (RLU) >1.4 were interpreted as positive.
    Abbott
    suggested: (Abbott, RRID:SCR_010477)
    The data were analyzed in GraphPad Prism (v9.0.2).
    GraphPad Prism
    suggested: (GraphPad Prism, RRID:SCR_002798)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:
    A limitation of this study is the small number of convalescent samples tested, and the absence of samples from vaccinated individuals, future work with more subjects, including vaccinated individuals will help to determine the clinical impact of this mutation, as well as its role in the effectiveness of currently approved vaccine strategies.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    Results from scite Reference Check: We found no unreliable references.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2021.09.13.21263430v1 on medRxiv