Inactivation of SARS-CoV-2 by β-propiolactone causes aggregation of viral particles and loss of antigenic potential

  1. Divya Gupta
  2. Haripriya Parthasarathy
  3. Vishal Sah
  4. Dixit Tandel
  5. Dhiviya Vedagiri
  6. Shashikala Reddy
  7. Krishnan H Harshan

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Abstract

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  1. Version published to 10.1016/j.virusres.2021.198555
  2. ScreenIT

    SciScore for 10.1101/2021.04.22.441045: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Ethicsnot detected.
    Sex as a biological variablenot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    Anti-Spike antibody was procured from Novus Biologicals (NB100-56578) while anti-Nucleocapsid was procured from Thermo Fisher (MA5-29982).
    Anti-Spike
    suggested: None
    anti-Nucleocapsid
    suggested: None
    HRP-conjugated anti-rabbit secondary antibody was purchased from Jackson ImmunoResearch (111-035-003).
    anti-rabbit
    suggested: (Santa Cruz Biotechnology Cat# sc-53804, RRID:AB_783976)
    Experimental Models: Cell Lines
    SentencesResources
    Cell culture and reagents: Vero cells were cultured in DMEM with 10% FBS (Hyclone, SH30084.03) and penicillin-streptomycin cocktail (Gibco, 15140-122) at 37°C and 5% CO2.
    Vero
    suggested: None
    Software and Algorithms
    SentencesResources
    Virus culturing: The oro- and nasopharyngeal patient swabs transported in VTM were screened using SARS-CoV-2 specific primers (LabGenomics; Labgun COVID-19 RT-PCR kit; CV9032B) and the samples with low Ct (less than 20) values were chosen to culture virus.
    SARS-CoV-2
    suggested: (BioLegend Cat# 944703, RRID:AB_2890874)
    All densitometric analyses were performed using ImageJ software [16].
    ImageJ
    suggested: (ImageJ, RRID:SCR_003070)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2021.04.22.441045v1 on bioRxiv