The DHODH inhibitor PTC299 arrests SARS-CoV-2 replication and suppresses induction of inflammatory cytokines

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1. Version published to 10.1016/j.virusres.2020.198246

   Jan 1, 2021
2. 

   ScreenIT

   Aug 6, 2020

   SciScore for 10.1101/2020.08.05.238394: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   , Vero (ATCC, CCL-81), Vero E6 cells (ATCC, CRL-1586) were maintained at 37°C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Hyclone or GIBCO) supplemented with 10% Fetal Bovine Serum (FBS, Corning or GIBCO).	Vero suggested: ATCC Cat# CRL-1586, RRID:CVCL_0574) Vero E6 suggested: None
   Huh7 cell line harboring a genotype 1b replicon was maintained under similar conditions but with the addition of 0.25 mg/ml G418 (GIBCO).	Huh7 suggested: …

   SciScore for 10.1101/2020.08.05.238394: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   , Vero (ATCC, CCL-81), Vero E6 cells (ATCC, CRL-1586) were maintained at 37°C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Hyclone or GIBCO) supplemented with 10% Fetal Bovine Serum (FBS, Corning or GIBCO).	Vero suggested: ATCC Cat# CRL-1586, RRID:CVCL_0574) Vero E6 suggested: None
   Huh7 cell line harboring a genotype 1b replicon was maintained under similar conditions but with the addition of 0.25 mg/ml G418 (GIBCO).	Huh7 suggested: None
   The received virus was amplified in HeLa cells, grown in DMEM supplemented with 5% FBS and 1% penicillin and streptomycin solution and the titer evaluated by plaque assay on HeLa cell monolayers.	HeLa suggested: CLS Cat# 300194/p772_HeLa, RRID:CVCL_0030)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: We found the following clinical trial numbers in your paper:
   

   Identifier	Status	Title
   NCT04439071	Recruiting	A Study to Evaluate Efficacy and Safety of PTC299 in Hospita…

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Read the original source
3. 

   Version published to 10.1101/2020.08.05.238394v1 on bioRxiv

   Aug 5, 2020