Cytoplasmic domain and enzymatic activity of ACE2 are not required for PI4KB dependent endocytosis entry of SARS-CoV-2 into host cells

  1. Hang Yang
  2. Huijun Yuan
  3. Xiaohui Zhao
  4. Meng Xun
  5. Shangrui Guo
  6. Nan Wang
  7. Bing Liu
  8. Hongliang Wang

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Abstract

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  1. Version published to 10.1016/j.virs.2022.03.003
  2. ScreenIT

    SciScore for 10.1101/2021.03.01.433503: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board Statementnot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    Antibodies and reagents: Antibodies used in this study include: SARS-CoV-2 spike (GeneTex, Taiwan), HA tag, EEA1 (Cell Signaling Technology, Danvers, MA), HIV-p24 (SinoBiological, Beijing, China)
    HA tag , EEA1 ( Cell Signaling Technology , Danvers , MA)
    suggested: None
    HIV-p24
    suggested: None
    PI4P and PI(4,5)P2 antibodies were purchased from Echelon Biosciences.
    PI(4,5)P2
    suggested: None
    Experimental Models: Cell Lines
    SentencesResources
    Cell lines and constructs: Huh 7, Vero E6 and 293T cells were grown in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (
    Vero E6
    suggested: RRID:CVCL_XD71)
    293T-ACE2 stable cell lines were generated by transfecting 293T cells with pSMPUM-ACE2 and selected with blasticidin.
    293T
    suggested: NCBI_Iran Cat# C498, RRID:CVCL_0063)
    For phosphatase inhibition test, 293T-ACE2 cells were first transfected with Sac1 or INPP5E.
    293T-ACE2
    suggested: RRID:CVCL_YZ65)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2021.03.01.433503v1 on bioRxiv