Adenovirus transduction to express human ACE2 causes obesity-specific morbidity in mice, impeding studies on the effect of host nutritional status on SARS-CoV-2 pathogenesis
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SciScore for 10.1101/2021.05.26.445786: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics IACUC: All animal handling protocols were approved by the Institutional Animal Care and Use Committee (Protocol #20-060) at Virginia Tech. Sex as a biological variable Mice and Diets: C57BL/6N male and female mice were obtained from Charles River Laboratories at three to four weeks of age and allowed to acclimatize for a week before initiating diets. Randomization not detected. Blinding The Virginia Tech Animal Laboratory Services (ViTALS) performed paraffin embedding, sectioning and hematoxylin-eosin staining, and a board-certified pathologist scored the slides in a blinded manner. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sente… SciScore for 10.1101/2021.05.26.445786: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics IACUC: All animal handling protocols were approved by the Institutional Animal Care and Use Committee (Protocol #20-060) at Virginia Tech. Sex as a biological variable Mice and Diets: C57BL/6N male and female mice were obtained from Charles River Laboratories at three to four weeks of age and allowed to acclimatize for a week before initiating diets. Randomization not detected. Blinding The Virginia Tech Animal Laboratory Services (ViTALS) performed paraffin embedding, sectioning and hematoxylin-eosin staining, and a board-certified pathologist scored the slides in a blinded manner. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources The viral stocks were propagated using 293A cells (ThermoFisher Catalog # R70507) and purified by cesium chloride gradient centrifugation prior to titering, also in 293A cells, as previously described [36]. 293Asuggested: RRID:CVCL_U006)Briefly, Vero E6 cells at 100% confluency were inoculated with 50 µL of the serially diluted samples and incubated at 37°C in 5% CO2 for 1 hour. Vero E6suggested: NoneExperimental Models: Organisms/Strains Sentences Resources Mice and Diets: C57BL/6N male and female mice were obtained from Charles River Laboratories at three to four weeks of age and allowed to acclimatize for a week before initiating diets. C57BL/6Nsuggested: RRID:MGI:5651595)Software and Algorithms Sentences Resources Statistical Analysis: Statistical analysis was done using GraphPad Prism 9 (GraphPad Software, San Diego, CA, USA). GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- No funding statement was detected.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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