Characterization of neutralizing versus binding antibodies and memory B cells in COVID-19 recovered individuals from India

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1. Version published to 10.1016/j.virol.2021.02.002

   Jun 1, 2021
2. 

   ScreenIT

   Sep 9, 2020

   SciScore for 10.1101/2020.08.31.276675: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IRB: The Institutional ethical boards approved the study. Consent: Informed consent was obtained prior to inclusion in the study.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   PBMC’s were stained with RBD-Alexa Fluor 488 for 1 hour at 4°C, followed by washing with PBS containing 0.25% FBS, and incubation with efluor780 Fixable Viability (Live Dead) dye (Life Technologies, #65-0865-14) and anti-human CD3, CD19, CD27, CD38 and IgD antibodies (BD Biosciences) for 30 minutes.	anti-human CD3 suggested: (RayBiotech Cat# …

   SciScore for 10.1101/2020.08.31.276675: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IRB: The Institutional ethical boards approved the study. Consent: Informed consent was obtained prior to inclusion in the study.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   PBMC’s were stained with RBD-Alexa Fluor 488 for 1 hour at 4°C, followed by washing with PBS containing 0.25% FBS, and incubation with efluor780 Fixable Viability (Live Dead) dye (Life Technologies, #65-0865-14) and anti-human CD3, CD19, CD27, CD38 and IgD antibodies (BD Biosciences) for 30 minutes.	anti-human CD3 suggested: (RayBiotech Cat# CS-11-0105, RRID:AB_1227994) CD19 suggested: (Agilent Cat# TC67401, RRID:AB_579635) CD27 , CD38 suggested: None IgD suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   This antibody-virus mixture was transferred into the wells of a 96-well plate that had been seeded with Vero-E6 cells the previous day at a concentration of 2.5× 104 cells/well.	Vero-E6 suggested: None
   Software and Algorithms
   Sentences	Resources
   Data was analyzed using FlowJo software 10.	FlowJo suggested: (FlowJo, RRID:SCR_008520)
   FRNT-mNG50 titers were calculated by non-linear regression analysis using the 4PL sigmoidal dose curve equation on Prism 8 (Graphpad Software).	Graphpad suggested: (GraphPad, RRID:SCR_000306)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • No conflict of interest statement was detected. If there are no conflicts, we encourage authors to explicit state so.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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3. 

   Version published to 10.1101/2020.08.31.276675v1 on bioRxiv

   Sep 1, 2020