Neutralizing antibody-dependent and -independent immune responses against SARS-CoV-2 in cynomolgus macaques
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Abstract
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SciScore for 10.1101/2020.08.18.256446: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IACUC: The protocols were approved by the Shiga University of Medical Science Animal Experiment Committee (permit number: 2020-4-2). Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable Animals: Fifteen-year-old and ten-year-old female cynomolgus macaques (CE0324F and CE1242F) and a fifteen-year-old male cynomolgus macaque (CE0202M) born at Shiga University of Medical Science were used. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Thawed cells (5 × 105/well) were cultured with a peptide pool of SARS-CoV-2 N protein (0.6 nmol/mL) (PepTivator, Miltenyi Biotech, Bergisch Gladbach, … SciScore for 10.1101/2020.08.18.256446: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IACUC: The protocols were approved by the Shiga University of Medical Science Animal Experiment Committee (permit number: 2020-4-2). Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable Animals: Fifteen-year-old and ten-year-old female cynomolgus macaques (CE0324F and CE1242F) and a fifteen-year-old male cynomolgus macaque (CE0202M) born at Shiga University of Medical Science were used. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Thawed cells (5 × 105/well) were cultured with a peptide pool of SARS-CoV-2 N protein (0.6 nmol/mL) (PepTivator, Miltenyi Biotech, Bergisch Gladbach, Germany) in the presence of anti-CD28 antibody (0.1 µg/mL) overnight in ELISPOT plates coated with anti-IFN-γ and IL-2 antibodies (Cellular Technology Limited, Shaker Heights, OH). anti-CD28suggested: Noneanti-IFN-γsuggested: NoneIL-2suggested: NoneExperimental Models: Cell Lines Sentences Resources VeroE6 cells were grown in MEM supplemented with 10% inactivated fetal bovine serum (Capricorn Scientific GmbH), VeroE6suggested: JCRB Cat# JCRB1819, RRID:CVCL_YQ49)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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