Oral subunit SARS-CoV-2 vaccine induces systemic neutralizing IgG, IgA and cellular immune responses and can boost neutralizing antibody responses primed by an injected vaccine
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SciScore for 10.1101/2021.06.09.447656: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics IRB: Protein production: Animal immunization: All animal studies were approved by the institutional Committee for Ethical Conduct in the Care and Use of Laboratory Animals and abided by guidelines set forth by the Animal Welfare Law Sex as a biological variable Assessment of humoral immune response: Assessment of cell-mediated immunity: Safety assessment of oral vaccine: Vaccine safety was determined by Envigo, Ness Ziona, Israel. Sprague Dawley rats (10-14 per treatment group, 8-weeks-old, equal number of male and females) were orally immunized 2 or 3 times at 14-day intervals with 100 μL MigVax-101 or with DP buffer as negative control. Randomization not detected. Blinding not detected. Power … SciScore for 10.1101/2021.06.09.447656: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics IRB: Protein production: Animal immunization: All animal studies were approved by the institutional Committee for Ethical Conduct in the Care and Use of Laboratory Animals and abided by guidelines set forth by the Animal Welfare Law Sex as a biological variable Assessment of humoral immune response: Assessment of cell-mediated immunity: Safety assessment of oral vaccine: Vaccine safety was determined by Envigo, Ness Ziona, Israel. Sprague Dawley rats (10-14 per treatment group, 8-weeks-old, equal number of male and females) were orally immunized 2 or 3 times at 14-day intervals with 100 μL MigVax-101 or with DP buffer as negative control. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
Experimental Models: Organisms/Strains Sentences Resources Assessment of humoral immune response: Assessment of cell-mediated immunity: Safety assessment of oral vaccine: Vaccine safety was determined by Envigo, Ness Ziona, Israel. Sprague Dawley rats (10-14 per treatment group, 8-weeks-old, equal number of male and females) were orally immunized 2 or 3 times at 14-day intervals with 100 μL MigVax-101 or with DP buffer as negative control. Sprague Dawleysuggested: NoneSoftware and Algorithms Sentences Resources All statistical analyses were performed using Prism (GraphPad). Prismsuggested: (PRISM, RRID:SCR_005375)GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:The limitations of this study included relatively diverse immune responses between animals, which may be attributed to the technical and physiological differences between animals at the time of administration, e.gsaliva conditions, technical oral delivery to the rodents, and others. Overcoming such obstacles may be achieved by improved formulation of the vaccine, enabling longer exposure to the vaccine. Taken together, the oral multi-epitope SARS-CoV-2 vaccine triggered versatile adaptive immune responses, which are expected to provide protection against viral infection and which should be useful for boosting immunity in those immunized with injected vaccines.
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- No funding statement was detected.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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