Recombinant SARS-CoV-2 spike S1-Fc fusion protein induced high levels of neutralizing responses in nonhuman primates
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SciScore for 10.1101/2020.04.21.052209: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable Female BALB/c mice were obtained from Vital River Co., China. New Zealand White rabbits were purchased and hosted at Longan Co., China. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Pseudovirus neutralization assay: HEK 293T cells and ACE2-transfected HEK 293T cells (ACE2-293T) were cultured in DMEM (Gibco, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Gibco), 50 U/ml penicillin (Gibco) and 50μg/ml streptomycin (Gibco) at 37°C with 5% CO2. HEK 293Tsuggested: NoneACE2-293T … SciScore for 10.1101/2020.04.21.052209: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable Female BALB/c mice were obtained from Vital River Co., China. New Zealand White rabbits were purchased and hosted at Longan Co., China. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Pseudovirus neutralization assay: HEK 293T cells and ACE2-transfected HEK 293T cells (ACE2-293T) were cultured in DMEM (Gibco, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Gibco), 50 U/ml penicillin (Gibco) and 50μg/ml streptomycin (Gibco) at 37°C with 5% CO2. HEK 293Tsuggested: NoneACE2-293T cells were seeded in 96-well plates at one day prior to infection. ACE2-293Tsuggested: NoneThe diluted serums were mixed with SARS-CoV-2 suspension of 100 TCID50 in 96-well plates at a ratio of 1:1, followed by 2 hours incubation at 36.5°C in a 5% CO2 incubator. 1-2 ×104 Vero cells were then added to the serum-virus mixture, and the plates were incubated for 5 days at 36.5°C in a 5% CO2 incubator. Verosuggested: NoneExperimental Models: Organisms/Strains Sentences Resources Female BALB/c mice were obtained from Vital River Co., China. New Zealand White rabbits were purchased and hosted at Longan Co., China. BALB/csuggested: RRID:IMSR_ORNL:BALB/cRl)Software and Algorithms Sentences Resources The 50% neutralization titer was calculated by probit analysis using the SPSS software. SPSSsuggested: (SPSS, RRID:SCR_002865)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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