Thermal analysis of protein stability and ligand binding in complex media
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SciScore for 10.1101/2021.12.01.470796: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Samples of the HEK293 cellular supernatant, Lot number: 03U27020D; unpurified angiotensin converting enzyme 2 (ACE2) expressed in human embryonic kidney cells (HEK293) received in the preparation media Lot number: 02U2802LL; isolated, purified ACE2 Lot number: 04U27020GC; and purified receptor binding domain (RBD) from SARS-CoV-2, Lot Number:05U22020TWB were purchased from RayBiotech (Peachtree Corners, GA). HEK293suggested: NoneThe HEK923 cell lysate was the … SciScore for 10.1101/2021.12.01.470796: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Samples of the HEK293 cellular supernatant, Lot number: 03U27020D; unpurified angiotensin converting enzyme 2 (ACE2) expressed in human embryonic kidney cells (HEK293) received in the preparation media Lot number: 02U2802LL; isolated, purified ACE2 Lot number: 04U27020GC; and purified receptor binding domain (RBD) from SARS-CoV-2, Lot Number:05U22020TWB were purchased from RayBiotech (Peachtree Corners, GA). HEK293suggested: NoneThe HEK923 cell lysate was the supernatant after initial centrifugation of the crude cell lysate and received in liquid form at unknown concentration. HEK923suggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- No funding statement was detected.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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