Refolding of lid subdomain of SARS-CoV-2 nsp14 upon nsp10 interaction releases exonuclease activity

  1. Anna Czarna
  2. Jacek Plewka
  3. Leanid Kresik
  4. Alex Matsuda
  5. Abdulkarim Karim
  6. Colin Robinson
  7. Sean O’Byrne
  8. Fraser Cunningham
  9. Irene Georgiou
  10. Piotr Wilk
  11. Magdalena Pachota
  12. Grzegorz Popowicz
  13. Paul Graham Wyatt
  14. Grzegorz Dubin
  15. Krzysztof Pyrć

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Abstract

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  1. Version published to 10.1016/j.str.2022.04.014
  2. ScreenIT

    SciScore for 10.1101/2022.02.17.480845: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Ethicsnot detected.
    Sex as a biological variablenot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.

    Table 2: Resources

    Recombinant DNA
    SentencesResources
    The sequence was subcloned into pETDuet-1 expression vector.
    pETDuet-1
    suggested: RRID:Addgene_170280)
    Software and Algorithms
    SentencesResources
    Restraints were obtained from ligand builder in Coot 19.
    Coot
    suggested: (Coot, RRID:SCR_014222)
    Data analysis: The final model was analyzed and the graphics was prepared using PyMOL (Schrödinger, LLC).
    PyMOL
    suggested: (PyMOL, RRID:SCR_000305)

    Results from OddPub: Thank you for sharing your data.

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    Results from scite Reference Check: We found no unreliable references.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2022.02.17.480845v1 on bioRxiv