Erythroid precursors and progenitors suppress adaptive immunity and get invaded by SARS-CoV-2

  1. Shima Shahbaz
  2. Lai Xu
  3. Mohammed Osman
  4. Wendy Sligl
  5. Justin Shields
  6. Michael Joyce
  7. D. Lorne Tyrrell
  8. Olaide Oyegbami
  9. Shokrollah Elahi

Reviewed by

Read the full article See related articles

Discuss this preprint

Start a discussion What are Sciety discussions?

Listed in

Log in to save this article

Abstract

No abstract available

Article activity feed

  1. Version published to 10.1016/j.stemcr.2021.04.001
  2. ScreenIT

    SciScore for 10.1101/2020.08.18.255927: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board StatementConsent: Waiver of consent was obtained by the HREB for those patients admitted to the ICU but a verbal consent was required from all other patients.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    For CECs or mock depletion samples were stained using anti-CD71 or isotype control biotin-conjugated antibody and fractioned using streptavidin linked magnetic beads (Miltenyi Biotec) according to our previous reports 24–26,31,34.
    anti-CD71
    suggested: None
    Specifically, the following antibodies were used: anti-CD3 (HIT3a), anti-CD4 (RPA-T4), anti-CD8 (RPA-T8), anti-CD45 (H-130 or 2D1), anti-VISTA (B7H5DS8), anti-107a (
    anti-CD3
    suggested: None
    anti-CD4
    suggested: (Thermo Fisher Scientific Cat# MA1-18657, RRID:AB_1072314)
    anti-CD8
    suggested: None
    anti-CD45
    suggested: None
    anti-VISTA
    suggested: None
    anti-107a
    suggested: None
    The membranes were blocked with 5% milk and incubated with anti-ACE2 (Abcam, ab15348), anti-TMPRSS2 (Abcam, ab242384) and anti-β-actin (Sigma, A2228) antibodies using dilutions 1:1,000.
    anti-ACE2
    suggested: (Abcam Cat# ab15348, RRID:AB_301861)
    anti-TMPRSS2
    suggested: (Abcam Cat# ab242384, RRID:AB_2889829)
    anti-β-actin
    suggested: (Sigma-Aldrich Cat# A2228, RRID:AB_476697)
    Experimental Models: Cell Lines
    SentencesResources
    HT29 (human colorectal adenocarcinoma grade II) whole cell lysate was used as a positive control for TMPRSS2 (Abcam, ab3952)
    HT29
    suggested: None
    Software and Algorithms
    SentencesResources
    Paraformaldehyde fixed cells were acquired by flow cytometry using a LSRFORTESSA flow cytometer (BD) and analyzed with FlowJo software.
    FlowJo
    suggested: (FlowJo, RRID:SCR_008520)
    Statistical analysis: Statistical comparisons between various groups were performed by using t-test and Mann-Whitney tests (as appropriate) using PRISM, Graph Pad software.
    PRISM
    suggested: (PRISM, RRID:SCR_005375)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:
    We are aware of the study limitations such as low sample size, especially in infected patients who do not require hospitalization. Moreover, due to blood sample size we were restricted in terms of the number and depth of analyses. For example, we would have liked to perform additional infection assays (e.g. drug titration, time points, blood group). Moreover, we were unable to perform longitudinal analyses of CECs functions/infectivity overtime. A larger sample would also be required to evaluate whether dexamethasone treatment reduces the viral load in patients (e.g. lungs) and if it influences the expression of ACE2 in different tissues.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2020.08.18.255927 on bioRxiv