SARS-CoV-2 Infects Human Pluripotent Stem Cell-Derived Cardiomyocytes, Impairing Electrical and Mechanical Function

SciScore for 10.1101/2020.08.30.274464: (What is this?)

Please note, not all rigor criteria are appropriate for all manuscripts.

Table 1: Rigor

Institutional Review Board Statement	not detected.
Randomization	not detected.
Blinding	not detected.
Power Analysis	not detected.
Sex as a biological variable	not detected.

Table 2: Resources

Antibodies
Sentences	Resources
Cells were centrifuged at 300 g for 5 min and incubated at room temperature for 1 h with either APC-cTnT antibody (Miltenyi Biotech #130-120-543) or APC-IgG1 isotype control antibody (Miltenyi Biotech #130-120-709), both used at 1:100 dilution in DPBS (Gibco) with 5% fetal bovine serum (FBS)and 0.75% saponin.	APC-IgG1 isotype control suggested: None
Primary antibodies were incubated in blocking buffer for 2 h at room temperature (rabbit anti-ACE2 [Abcam #ab15348, used at 1:500 dilution]; mouse …

SciScore for 10.1101/2020.08.30.274464: (What is this?)

Please note, not all rigor criteria are appropriate for all manuscripts.

Table 1: Rigor

Institutional Review Board Statement	not detected.
Randomization	not detected.
Blinding	not detected.
Power Analysis	not detected.
Sex as a biological variable	not detected.

Table 2: Resources

Antibodies
Sentences	Resources
Cells were centrifuged at 300 g for 5 min and incubated at room temperature for 1 h with either APC-cTnT antibody (Miltenyi Biotech #130-120-543) or APC-IgG1 isotype control antibody (Miltenyi Biotech #130-120-709), both used at 1:100 dilution in DPBS (Gibco) with 5% fetal bovine serum (FBS)and 0.75% saponin.	APC-IgG1 isotype control suggested: None
Primary antibodies were incubated in blocking buffer for 2 h at room temperature (rabbit anti-ACE2 [Abcam #ab15348, used at 1:500 dilution]; mouse anti-GAPDH [Abcam #ab8245, used at 1:3,000 dilution]).	anti-ACE2 suggested: (Abcam Cat# ab15348, RRID:AB_301861) anti-GAPDH suggested: (Abcam Cat# ab8245, RRID:AB_2107448)
Membranes were washed and further incubated with fluorescent dye-conjugated secondary antibodies for 1 h at room temperature (AlexaFluor 647 goat anti-rabbit IgG1 and AlexaFluor 488 goat anti-mouse IgG1, both used at 1:1,000 dilution in blocking buffer) and fluorescent signals were acquired using with a GelDoc Imager (Bio-Rad)	anti-rabbit IgG1 suggested: None anti-mouse IgG1 suggested: None
Primary antibodies were incubated overnight at 4 °C in DPBS with 1% normal goat serum and 0.1% Tween-20 (rabbit anti-2019-nCoV NP [Sino Biological #40143-R019, used at 1:200 dilution]; and mouse anti-Sarcomeric α-actinin [Abcam ab# ab9465, used at 1:500 dilution]).	anti-2019-nCoV NP suggested: None anti-Sarcomeric α-actinin suggested: None
Software and Algorithms
Sentences	Resources
On day 6 media was changed to RPMI-1640 plus B-27 supplement (ThermoFisher), with further media changes every other day.	ThermoFisher suggested: (ThermoFisher; SL 8; Centrifuge, RRID:SCR_020809)
Following washes with DPBS with 5% FBS, samples were run on a BD FACSCanto II flow cytometer and data from 10,000 valid events were acquired with the BD FACSDIVA software.	BD FACSDIVA suggested: (BD FACSDiva Software, RRID:SCR_001456)
Analysis was performed with FlowJo v10.7.	FlowJo suggested: (FlowJo, RRID:SCR_008520)
The FASTQ files were separately mapped to the GRC38 human reference genome using STAR as a part of the cellranger pipeline.	STAR suggested: (STAR, RRID:SCR_015899)
Gene expression counts were done using cellranger count based on Gencode v25 annotation, and cell identifiers and Unique Molecular Identifiers (UMI) were filtered and corrected with default setting.	Gencode suggested: (GENCODE, RRID:SCR_014966)
Images were taken with a 40x oil objective on a Nikon Eclipse microscope with Yokogawa W1 spinning disk head, and formatted with Fiji software.	Fiji suggested: (Fiji, RRID:SCR_002285)
Electrophysiological recordings were taken for 5 min at specified time points using Axis software version 2.0.4.	Axis suggested: (AxIS , RRID:SCR_016308)
Data from the PCB was collected by LabView (National Instruments) on a laptop in the BSL-3 facility.	LabView suggested: (LabView , RRID:SCR_014325)
The voltage traces from the magnetic sensors were analyzed for amplitude and frequency using a custom Matlab protocol.	Matlab suggested: (MATLAB, RRID:SCR_001622)
Statistical analyses: Statistical analyses were performed using Prism 8.1.3 (GraphPad).	Prism suggested: (PRISM, RRID:SCR_005375) GraphPad suggested: (GraphPad Prism, RRID:SCR_002798)

Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

Results from TrialIdentifier: No clinical trial numbers were referenced.

Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).

Results from JetFighter: We did not find any issues relating to colormaps.

Results from rtransparent:

Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
No protocol registration statement was detected.

Read the original source

SciScore for 10.1101/2020.08.30.274464: (What is this?)

Please note, not all rigor criteria are appropriate for all manuscripts.

Table 1: Rigor

Institutional Review Board Statement

not detected.

Randomization

not detected.

Blinding

not detected.

Power Analysis

not detected.

Sex as a biological variable

not detected.

Table 2: Resources

Antibodies
Sentences	Resources
Cells were centrifuged at 300 g for 5 min and incubated at room temperature for 1 h with either APC-cTnT antibody (Miltenyi Biotech #130-120-543) or APC-IgG1 isotype control antibody (Miltenyi Biotech #130-120-709), both used at 1:100 dilution in DPBS (Gibco) with 5% fetal bovine serum (FBS) and 0.75% saponin.	APC-IgG1 isotype control suggested: None
Primary antibodies were incubated in blocking buffer for 2 h at room temperature (rabbit anti-ACE2 [Abcam #ab15348, used at 1:500 dilution]; mouse anti-GAPDH [Abcam #ab8245, used at 1:3,000 dilution]).	anti-ACE2 suggested: (Abcam Cat# ab15348, RRID:AB_301861) anti-GAPDH suggested: (Abcam Cat# ab8245, RRID:AB_2107448)
Membranes were washed and further incubated with fluorescent dye-conjugated secondary antibodies for 1 h at room temperature (AlexaFluor 647 goat anti-rabbit IgG1 and AlexaFluor 488 goat anti-mouse IgG1, both used at 1:1,000 dilution in blocking buffer) and fluorescent signals were acquired using with a GelDoc Imager (Bio-Rad)	anti-rabbit IgG1 suggested: None anti-mouse IgG1 suggested: None
Primary antibodies were incubated overnight at 4 °C in DPBS with 1% normal goat serum and 0.1% Tween-20 (rabbit anti2019-nCoV NP [Sino Biological #40143-R019, used at 1:200 dilution]; and mouse anti-Sarcomeric α-actinin [Abcam ab# ab9465, used at 1:500 dilution]).	anti2019-nCoV NP suggested: None anti-Sarcomeric α-actinin suggested: None
Software and Algorithms
Sentences	Resources
Following washes with DPBS with 5% FBS, samples were run on a BD FACSCanto II flow cytometer and data from 10,000 valid events were acquired with the BD FACSDIVA software.	BD FACSDIVA suggested: (BD FACSDiva Software, RRID:SCR_001456)
Analysis was performed with FlowJo v10.7.	FlowJo suggested: (FlowJo, RRID:SCR_008520)
The FASTQ files were separately mapped to the GRC38 human reference genome using STAR as a part of the cellranger pipeline.	STAR suggested: (STAR, RRID:SCR_015899)
Gene expression counts were done using cellranger count based on Gencode v25 annotation, and cell identifiers and Unique Molecular Identifiers (UMI) were filtered and corrected with default setting.	Gencode suggested: (GENCODE, RRID:SCR_014966)
Images were taken with a 40x oil objective on a Nikon Eclipse microscope with Yokogawa W1 spinning disk head, and formatted with Fiji software.	Fiji suggested: (Fiji, RRID:SCR_002285)
Electrophysiological recordings were taken for 5 min at specified time points using Axis software version 2.0.4.	Axis suggested: (AxIS , RRID:SCR_016308)
Data from the PCB was collected by LabView (National Instruments) on a laptop in the BSL-3 facility.	LabView suggested: (LabView , RRID:SCR_014325)
The voltage traces from the magnetic sensors were analyzed for amplitude and frequency using a custom Matlab protocol.	Matlab suggested: (MATLAB, RRID:SCR_001622)
Statistical analyses were performed using Prism 8.1.3 (GraphPad).	Prism suggested: (PRISM, RRID:SCR_005375) GraphPad suggested: (GraphPad Prism, RRID:SCR_002798)

Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

Results from TrialIdentifier: No clinical trial numbers were referenced.

Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).

Results from JetFighter: We did not find any issues relating to colormaps.

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SARS-CoV-2 Infects Human Pluripotent Stem Cell-Derived Cardiomyocytes, Impairing Electrical and Mechanical Function

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