SARS-CoV-2 Infects Human Pluripotent Stem Cell-Derived Cardiomyocytes, Impairing Electrical and Mechanical Function
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SciScore for 10.1101/2020.08.30.274464: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Antibodies Sentences Resources Cells were centrifuged at 300 g for 5 min and incubated at room temperature for 1 h with either APC-cTnT antibody (Miltenyi Biotech #130-120-543) or APC-IgG1 isotype control antibody (Miltenyi Biotech #130-120-709), both used at 1:100 dilution in DPBS (Gibco) with 5% fetal bovine serum (FBS)and 0.75% saponin. APC-IgG1 isotype controlsuggested: NonePrimary antibodies were incubated in blocking buffer for 2 h at room temperature (rabbit anti-ACE2 [Abcam #ab15348, used at 1:500 dilution]; mouse … SciScore for 10.1101/2020.08.30.274464: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Antibodies Sentences Resources Cells were centrifuged at 300 g for 5 min and incubated at room temperature for 1 h with either APC-cTnT antibody (Miltenyi Biotech #130-120-543) or APC-IgG1 isotype control antibody (Miltenyi Biotech #130-120-709), both used at 1:100 dilution in DPBS (Gibco) with 5% fetal bovine serum (FBS)and 0.75% saponin. APC-IgG1 isotype controlsuggested: NonePrimary antibodies were incubated in blocking buffer for 2 h at room temperature (rabbit anti-ACE2 [Abcam #ab15348, used at 1:500 dilution]; mouse anti-GAPDH [Abcam #ab8245, used at 1:3,000 dilution]). anti-ACE2suggested: (Abcam Cat# ab15348, RRID:AB_301861)anti-GAPDHsuggested: (Abcam Cat# ab8245, RRID:AB_2107448)Membranes were washed and further incubated with fluorescent dye-conjugated secondary antibodies for 1 h at room temperature (AlexaFluor 647 goat anti-rabbit IgG1 and AlexaFluor 488 goat anti-mouse IgG1, both used at 1:1,000 dilution in blocking buffer) and fluorescent signals were acquired using with a GelDoc Imager (Bio-Rad) anti-rabbit IgG1suggested: Noneanti-mouse IgG1suggested: NonePrimary antibodies were incubated overnight at 4 °C in DPBS with 1% normal goat serum and 0.1% Tween-20 (rabbit anti-2019-nCoV NP [Sino Biological #40143-R019, used at 1:200 dilution]; and mouse anti-Sarcomeric α-actinin [Abcam ab# ab9465, used at 1:500 dilution]). anti-2019-nCoV NPsuggested: Noneanti-Sarcomeric α-actininsuggested: NoneSoftware and Algorithms Sentences Resources On day 6 media was changed to RPMI-1640 plus B-27 supplement (ThermoFisher), with further media changes every other day. ThermoFishersuggested: (ThermoFisher; SL 8; Centrifuge, RRID:SCR_020809)Following washes with DPBS with 5% FBS, samples were run on a BD FACSCanto II flow cytometer and data from 10,000 valid events were acquired with the BD FACSDIVA software. BD FACSDIVAsuggested: (BD FACSDiva Software, RRID:SCR_001456)Analysis was performed with FlowJo v10.7. FlowJosuggested: (FlowJo, RRID:SCR_008520)The FASTQ files were separately mapped to the GRC38 human reference genome using STAR as a part of the cellranger pipeline. STARsuggested: (STAR, RRID:SCR_015899)Gene expression counts were done using cellranger count based on Gencode v25 annotation, and cell identifiers and Unique Molecular Identifiers (UMI) were filtered and corrected with default setting. Gencodesuggested: (GENCODE, RRID:SCR_014966)Images were taken with a 40x oil objective on a Nikon Eclipse microscope with Yokogawa W1 spinning disk head, and formatted with Fiji software. Fijisuggested: (Fiji, RRID:SCR_002285)Electrophysiological recordings were taken for 5 min at specified time points using Axis software version 2.0.4. Axissuggested: (AxIS , RRID:SCR_016308)Data from the PCB was collected by LabView (National Instruments) on a laptop in the BSL-3 facility. LabViewsuggested: (LabView , RRID:SCR_014325)The voltage traces from the magnetic sensors were analyzed for amplitude and frequency using a custom Matlab protocol. Matlabsuggested: (MATLAB, RRID:SCR_001622)Statistical analyses: Statistical analyses were performed using Prism 8.1.3 (GraphPad). Prismsuggested: (PRISM, RRID:SCR_005375)GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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SciScore for 10.1101/2020.08.30.274464: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Antibodies Sentences Resources Cells were centrifuged at 300 g for 5 min and incubated at room temperature for 1 h with either APC-cTnT antibody (Miltenyi Biotech #130-120-543) or APC-IgG1 isotype control antibody (Miltenyi Biotech #130-120-709), both used at 1:100 dilution in DPBS (Gibco) with 5% fetal bovine serum (FBS) and 0.75% saponin. APC-IgG1 isotype controlsuggested: NonePrimary antibodies were … SciScore for 10.1101/2020.08.30.274464: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Antibodies Sentences Resources Cells were centrifuged at 300 g for 5 min and incubated at room temperature for 1 h with either APC-cTnT antibody (Miltenyi Biotech #130-120-543) or APC-IgG1 isotype control antibody (Miltenyi Biotech #130-120-709), both used at 1:100 dilution in DPBS (Gibco) with 5% fetal bovine serum (FBS) and 0.75% saponin. APC-IgG1 isotype controlsuggested: NonePrimary antibodies were incubated in blocking buffer for 2 h at room temperature (rabbit anti-ACE2 [Abcam #ab15348, used at 1:500 dilution]; mouse anti-GAPDH [Abcam #ab8245, used at 1:3,000 dilution]). anti-ACE2suggested: (Abcam Cat# ab15348, RRID:AB_301861)anti-GAPDHsuggested: (Abcam Cat# ab8245, RRID:AB_2107448)Membranes were washed and further incubated with fluorescent dye-conjugated secondary antibodies for 1 h at room temperature (AlexaFluor 647 goat anti-rabbit IgG1 and AlexaFluor 488 goat anti-mouse IgG1, both used at 1:1,000 dilution in blocking buffer) and fluorescent signals were acquired using with a GelDoc Imager (Bio-Rad) anti-rabbit IgG1suggested: Noneanti-mouse IgG1suggested: NonePrimary antibodies were incubated overnight at 4 °C in DPBS with 1% normal goat serum and 0.1% Tween-20 (rabbit anti2019-nCoV NP [Sino Biological #40143-R019, used at 1:200 dilution]; and mouse anti-Sarcomeric α-actinin [Abcam ab# ab9465, used at 1:500 dilution]). anti2019-nCoV NPsuggested: Noneanti-Sarcomeric α-actininsuggested: NoneSoftware and Algorithms Sentences Resources Following washes with DPBS with 5% FBS, samples were run on a BD FACSCanto II flow cytometer and data from 10,000 valid events were acquired with the BD FACSDIVA software. BD FACSDIVAsuggested: (BD FACSDiva Software, RRID:SCR_001456)Analysis was performed with FlowJo v10.7. FlowJosuggested: (FlowJo, RRID:SCR_008520)The FASTQ files were separately mapped to the GRC38 human reference genome using STAR as a part of the cellranger pipeline. STARsuggested: (STAR, RRID:SCR_015899)Gene expression counts were done using cellranger count based on Gencode v25 annotation, and cell identifiers and Unique Molecular Identifiers (UMI) were filtered and corrected with default setting. Gencodesuggested: (GENCODE, RRID:SCR_014966)Images were taken with a 40x oil objective on a Nikon Eclipse microscope with Yokogawa W1 spinning disk head, and formatted with Fiji software. Fijisuggested: (Fiji, RRID:SCR_002285)Electrophysiological recordings were taken for 5 min at specified time points using Axis software version 2.0.4. Axissuggested: (AxIS , RRID:SCR_016308)Data from the PCB was collected by LabView (National Instruments) on a laptop in the BSL-3 facility. LabViewsuggested: (LabView , RRID:SCR_014325)The voltage traces from the magnetic sensors were analyzed for amplitude and frequency using a custom Matlab protocol. Matlabsuggested: (MATLAB, RRID:SCR_001622)Statistical analyses were performed using Prism 8.1.3 (GraphPad). Prismsuggested: (PRISM, RRID:SCR_005375)GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
About SciScore
SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.
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