Tracking the temporal variation of COVID-19 surges through wastewater-based epidemiology during the peak of the pandemic: A six-month long study in Charlotte, North Carolina
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SciScore for 10.1101/2021.09.23.21258047: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Field Sample Permit: Deionized water in a 1 L Nalgene sample collection bottle was used as a field blank. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources 2.4.7 N1 and N2 Standard: Armored RNA Quant SARS-CoV-2 control, which encapsulates the in vitro transcribed RNA template in a protective protein coat and targets the SARS-CoV-2 viral nucleocapsid (N) region, was used as a positive control and run in duplicate for every set of reactions targeting N1 and N2. 2.4.8 Limit of Detection, Limit of Quantification, and Limit of … SciScore for 10.1101/2021.09.23.21258047: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Field Sample Permit: Deionized water in a 1 L Nalgene sample collection bottle was used as a field blank. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources 2.4.7 N1 and N2 Standard: Armored RNA Quant SARS-CoV-2 control, which encapsulates the in vitro transcribed RNA template in a protective protein coat and targets the SARS-CoV-2 viral nucleocapsid (N) region, was used as a positive control and run in duplicate for every set of reactions targeting N1 and N2. 2.4.8 Limit of Detection, Limit of Quantification, and Limit of Blank: For the determination of LoD using RT-ddPCR, the Limit of Blank (LoB) was elucidated from eight replicates of negative matrix samples derived from influent collected at multiple WWTP throughout eastern NC. N1suggested: RRID:CVCL_Z404)Experimental Models: Organisms/Strains Sentences Resources 2.4.2 Detection and quantification using RT-ddPCR: RT-ddPCR was utilized to quantify SARS-CoV-2 RNA copies targeting N1 and N2, described previously (Table S1), and utilizing a two-step reverse transcription and RT-ddPCR. N2suggested: NoneSoftware and Algorithms Sentences Resources RT-qPCR runs were analyzed by Bio-Rad CFX Manager software version 3.1 (Bio-Rad Laboratories). Bio-Rad CFX Managersuggested: NoneCFXsuggested: NoneBio-Rad Laboratoriessuggested: (Bio-Rad Laboratories, RRID:SCR_008426)KingFisher script is provided in the supplementary material (Table S7a). KingFishersuggested: (Hamilton NIMBUS Presto and ID NIMBUS Presto Assay Ready Workstation, RRID:SCR_019998)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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