Prevalence and stability of SARS-CoV-2 RNA on Bangladeshi banknotes

  1. Selina Akter
  2. Pravas Chandra Roy
  3. Amina Ferdaus
  4. Habiba Ibnat
  5. A.S.M. Rubayet Ul Alam
  6. Shireen Nigar
  7. Iqbal Kabir Jahid
  8. M. Anwar Hossain

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Abstract

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  1. Version published to 10.1016/j.scitotenv.2021.146133
  2. ScreenIT

    SciScore for 10.1101/2020.11.26.20233627: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board StatementIRB: 2.1 Ethics approval: The work has been conducted in the Genome Centre of Jashore University of Science and Technology, which is providing SARS-CoV-2 real time RT PCR diagnostic service as part of national COVID 19 response and surveillance program (https://dghs.gov.bd/images/docs/Notice/rt_pcr_lab.pdf).
    Consent: The research project has been reviewed by the institutional ethical review board of Jashore University of Science and Technology with an exemption of consent from the patients (ref: ERC/FBS/JUST/2020-42).
    Randomization2.7 Targeted (Sanger) sequencing of SARS-CoV-2 gene: The randomly amplified cDNAs were used as template for the PCR targeting N protein coding sequence for detecting the viral phylogenetic clade (M. T. Islam et al., 2020).
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.

    Table 2: Resources

    Software and Algorithms
    SentencesResources
    Both the sides of the banknote was swabed with a nylon flocked round (oral) swab (Biovencer Healthcare Pvt. ltd.,
    Biovencer Healthcare
    suggested: None
    CT values from the stability on banknotes have been transformed as 1/CT*100 and were plotted with respective time (hr) by using Microsoft Excel 2010Add-in GInaFiT 1.6 (Geeraerd et al., 2005) (https://cit.kuleuven.be/biotec/software/GinaFit).
    Microsoft Excel
    suggested: (Microsoft Excel, RRID:SCR_016137)
    The α, □1, ρ, □2 values were analyzed by carrying out ANOVA by using SAS software version 9.2 (SAS Institute Inc.
    SAS
    suggested: (SASqPCR, RRID:SCR_003056)
    SAS Institute
    suggested: (Statistical Analysis System, RRID:SCR_008567)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2020.11.26.20233627v1 on medRxiv