First detection of SARS-CoV-2 genetic material in the vicinity of COVID-19 isolation Centre in Bangladesh: Variation along the sewer network
This article has been Reviewed by the following groups
Listed in
- Evaluated articles (ScreenIT)
Abstract
Article activity feed
-
-
SciScore for 10.1101/2020.09.14.20194696: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
No key resources detected.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:Further, referring to the limitations of the present study, surrogate samples were sent to other laboratories to check how precisely the Sansure kit data matches with others. We also tested the filtration methods to check with …
SciScore for 10.1101/2020.09.14.20194696: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
No key resources detected.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:Further, referring to the limitations of the present study, surrogate samples were sent to other laboratories to check how precisely the Sansure kit data matches with others. We also tested the filtration methods to check with the efficiency of the PEG method used in this study. Although, Hata and Honda (2020) reported a high efficiency of the PEG method in Japanese wastewater, and the same has been found true by Kumar et al. (2020) while testing the sample concentration efficiency between PEG and filtration. While lack of supply always poses critical challenges in any research, during lockdown we could not find a supply of MS2, as used by Kumar et al. (2020) or could perform the whole process control (WPC) together with MPC as recommended by Haramoto et al. (2020). We had to use a swab sample of a symptomatic person as our control as indicated by CY-5 as quality control of our analyses, which makes sense and provides a low-cost control for such analyses, yet it may be controversial, less precise and at times comes negative as the case in a few samples in our case (drain 1 samples at 300 and 400m and main sewer sample on 07th and 26th August). By applying fluorescence quantitative RT-PCR technology, this test utilized the novel Covid-19 ORF1ab and the specific conserved sequence of coding nucleocapsid protein N gene as the target regions, which were designed for conserved sequences of the double target genes to achieve detection of wastewater samples RNA through fluorescent s...
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
-
-