SARS-CoV-2 has been circulating in northern Italy since December 2019: Evidence from environmental monitoring

  1. Giuseppina La Rosa
  2. Pamela Mancini
  3. Giusy Bonanno Ferraro
  4. Carolina Veneri
  5. Marcello Iaconelli
  6. Lucia Bonadonna
  7. Luca Lucentini
  8. Elisabetta Suffredini

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Abstract

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  1. Version published to 10.1016/j.scitotenv.2020.141711
  2. ScreenIT

    SciScore for 10.1101/2020.06.25.20140061: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board Statementnot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.

    Table 2: Resources

    Software and Algorithms
    SentencesResources
    PCR reaction was performed using 2.5 μl of cDNA in a final volume of 25 μl (Kit Platinum SuperFi Green PCR Master Mix, Thermo), using 1 μl of the primer (10 μM).
    Thermo
    suggested: (Thermo Xcalibur, RRID:SCR_014593)
    Sequences were identified using BLAST analysis (https://blast.ncbi.nlm.nih.gov/Blast.cgi).
    BLAST
    suggested: (BLASTX, RRID:SCR_001653)
    https://blast.ncbi.nlm.nih.gov/Blast.cgi
    suggested: (TBLASTX, RRID:SCR_011823)
    For comparison purposes, all Italian SARS-CoV-2 genome sequences available at the time of analysis (12th June 2020; n=134) were retrieved from Gisaid (https://www.gisaid.org/) and aligned with the study sequences using the MEGA X software (Kumar et al., 2018).
    MEGA X
    suggested: None

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2020.06.25.20140061v1 on medRxiv