Impact of various vaccine boosters on neutralization against omicron following prime vaccinations with inactivated or adenovirus-vectored vaccine

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1. Version published to 10.1016/j.scib.2022.05.010

   Jul 1, 2022
2. 

   ScreenIT

   Jan 31, 2022

   SciScore for 10.1101/2022.01.25.476850: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics

   Field Sample Permit: Human serum samples: Human serum specimens were collected at Peking Union Medical College Hospital, Beijing Ditan Hospital, 309 Hospital of the Chinese People’s Liberation Army, Fangzhuang Community Health Service Center, and the Institute of Microbiology of the Chinese Academy of Sciences (IMCAS). IRB: This study was conducted in strict accordance with the recommendations in the “Guide for the Care and Use of Laboratory Animals” issued by the Ethics Committee of IMCAS. Consent: Informed consent was obtained from all of the participants.

   Sex as a biological variable

   Specific-pathogen-free (SPF) BALB/c 6-8-week-old female mice were purchased from Beijing Vital River Animal …

   SciScore for 10.1101/2022.01.25.476850: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	Field Sample Permit: Human serum samples: Human serum specimens were collected at Peking Union Medical College Hospital, Beijing Ditan Hospital, 309 Hospital of the Chinese People’s Liberation Army, Fangzhuang Community Health Service Center, and the Institute of Microbiology of the Chinese Academy of Sciences (IMCAS). IRB: This study was conducted in strict accordance with the recommendations in the “Guide for the Care and Use of Laboratory Animals” issued by the Ethics Committee of IMCAS. Consent: Informed consent was obtained from all of the participants.
   Sex as a biological variable	Specific-pathogen-free (SPF) BALB/c 6-8-week-old female mice were purchased from Beijing Vital River Animal Technology Co.,
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   The HRP-labeled anti-mouse Fc secondary antibody (Yeasen) was added after washing the microplates three times.	anti-mouse suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   Protein expression and purification: The objective recombinant protein sequences carried on the pCAGGS vector were expressed via HEK293T cells.	HEK293T suggested: KCB Cat# KCB 200744YJ, RRID:CVCL_0063)
   After incubation at 37°C for one hour, 2 × 104 Vero E6 per cell were added to each well to make a final volume of 200 μl, which was incubated for 24 hours in a 37°C, 5% CO2 incubator.	Vero E6 suggested: None
   Experimental Models: Organisms/Strains
   Sentences	Resources
   Animal experiments: For immunization, 6-8-week-old female BALB/c mice were primarily vaccinated with 4 μg S-encoding mRNA vaccine candidate via intramuscular (i.m.) route.	BALB/c suggested: RRID:IMSR_ORNL:BALB/cRl)
   Recombinant DNA
   Sentences	Resources
   Protein expression and purification: The objective recombinant protein sequences carried on the pCAGGS vector were expressed via HEK293T cells.	pCAGGS suggested: RRID:Addgene_127347)
   Software and Algorithms
   Sentences	Resources
   All of the graphs were generated with GraphPad Prism version 7.0 software.	GraphPad Prism suggested: (GraphPad Prism, RRID:SCR_002798)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Results from scite Reference Check: We found no unreliable references.

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   Read the original source
3. 

   Version published to 10.1101/2022.01.25.476850v1 on bioRxiv

   Jan 27, 2022