Preclinical study of a DNA vaccine targeting SARS-CoV-2
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SciScore for 10.1101/2020.10.21.347799: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IACUC: All experiments were approved by the Ethical Committee for Animal Experiments of the Osaka University Graduate School of Medicine.
Consent: The use of patients sera with written informed consent was approved by an Ethics Committee of Research Instituted for Microbial Diseases Osaka University (approval number 31-14-1).
IRB: The use of patients sera with written informed consent was approved by an Ethics Committee of Research Instituted for Microbial Diseases Osaka University (approval number 31-14-1).Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable Animal protocol: Seven-week-old male Sprague-Dawley (SD) … SciScore for 10.1101/2020.10.21.347799: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IACUC: All experiments were approved by the Ethical Committee for Animal Experiments of the Osaka University Graduate School of Medicine.
Consent: The use of patients sera with written informed consent was approved by an Ethics Committee of Research Instituted for Microbial Diseases Osaka University (approval number 31-14-1).
IRB: The use of patients sera with written informed consent was approved by an Ethics Committee of Research Instituted for Microbial Diseases Osaka University (approval number 31-14-1).Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable Animal protocol: Seven-week-old male Sprague-Dawley (SD) rats were purchased from Clea Japan Inc. (Tokyo, Japan), and housed with free access to food and water in a temperature and light cycle-controlled facility. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources After the membrane was blocked with PBS containing 5 % skim milk for 1 hour at room temperature (RT), the membrane was incubated with an antibody against SARS-CoV-2 Spike (GNX135356, GeneTex, Inc.) at 4 °C overnight. antibody against SARS-CoV-2 Spike ( GNX135356suggested: NoneSandwich ELISA for detection of secreted spike protein: A diluted capture antibody for the spike protein (GTX632604, GeneTex) was coated on a 96-well plate and incubated at 4 °C overnight. GTX632604suggested: (GeneTex Cat# GTX632604, RRID:AB_2864418)Ninety-six-well PVDF membrane-bottomed plates (Merck Millipore) were coated with an anti-rat IFNγ capture antibody or IL4 capture antibody and incubated at 4 °C overnight. anti-rat IFNγsuggested: NoneThe washed plates were incubated with a biotinylated polyclonal antibody specific for rat IFNγ or IL4 for 2 hours at 4 °C. IL4suggested: NoneAfter washing with PBS-T, plate was incubated with anti-his antibody conjugated with HRP (abcam) for 2 h at RT. anti-hissuggested: NoneAs first antibody, 1μg/ml of anti-SARS-CoV/SARS-CoV-2 nucleoprotein monoclonal antibody, HM1054 (EastCoast Bio) was used. anti-SARS-CoV/SARS-CoV-2 nucleoproteinsuggested: NoneExperimental Models: Cell Lines Sentences Resources Cells, DNA transfection, western blotting, and immunostaining: Human embryonic kidney 293 (HEK293) cells were cultured in DMEM containing 10 % FBS plus penicillin/streptomycin and grown at 37 °C in a humidified 5 % CO2 incubator. HEK293suggested: NonePseudoviruses were incubated with a series of dilutions of inactivated rat serum for 1 hour at 37 °C, and then added to Vero E6 cells. Vero E6suggested: RRID:CVCL_XD71)VeroE6/TMPRSS2 cells (4×104 cells/well) were seeded on 96-well plates and incubated at 37° for 24 hours. VeroE6/TMPRSS2suggested: JCRB Cat# JCRB1819, RRID:CVCL_YQ49)Experimental Models: Organisms/Strains Sentences Resources Animal protocol: Seven-week-old male Sprague-Dawley (SD) rats were purchased from Clea Japan Inc. (Tokyo, Japan), and housed with free access to food and water in a temperature and light cycle-controlled facility. Sprague-Dawleysuggested: RRID:RGD_70508)Software and Algorithms Sentences Resources The value of the half-maximal antibody titer of each sample was calculated from the highest absorbance in the dilution range by using Prism 6 software. Prismsuggested: (PRISM, RRID:SCR_005375)Signals were detected with a ChemiDoc Touch Imaging System (Bio-Rad) and analyzed with Image Lab software version 6.0.1 ( Image Labsuggested: (Image Lab Software, RRID:SCR_014210)One-way ANOVA followed by Tukey’s post hoc test was used to assess significant differences in each experiment using Prism 6 software (GraphPad Software). GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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