In silico, in vitro screening of plant extracts for anti-SARS-CoV-2 activity and evaluation of their acute and sub-acute toxicity
This article has been Reviewed by the following groups
Listed in
- Evaluated articles (ScreenIT)
Abstract
Article activity feed
-
-
SciScore for 10.1101/2021.09.07.459230: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Field Sample Permit: Experimental animals: Toxicity assays were conducted in accordance with the standard guidelines of the Organization for Economic Cooperation and Development (OECD, 2001) for use of animals in scientific research. Sex as a biological variable The experimental female rats were bred and reared at the animal care facility in NUCARE, Nitte University, Mangalore and experiment was carried out in the same facility. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources 1x10e4 VeroE6 cells were plated per well and incubated at 37-degree C overnight for the … SciScore for 10.1101/2021.09.07.459230: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Field Sample Permit: Experimental animals: Toxicity assays were conducted in accordance with the standard guidelines of the Organization for Economic Cooperation and Development (OECD, 2001) for use of animals in scientific research. Sex as a biological variable The experimental female rats were bred and reared at the animal care facility in NUCARE, Nitte University, Mangalore and experiment was carried out in the same facility. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources 1x10e4 VeroE6 cells were plated per well and incubated at 37-degree C overnight for the monolayer formation. VeroE6suggested: JCRB Cat# JCRB1819, RRID:CVCL_YQ49)Experimental Models: Organisms/Strains Sentences Resources Adult albino Wistar non-pregnant female rats weighing 200−220 g and between 8–10 weeks of age were used. 2.4.2. Wistarsuggested: NoneSoftware and Algorithms Sentences Resources The structure of plant molecules were sketched using a 2D sketcher tool available in Schrodinger maestro and subjected for ligand minimisation using Ligprep Ligprepsuggested: (Ligprep, RRID:SCR_016746)The docking of phytochemicals over target proteins was performed using Glide v7.8. Glidesuggested: (Glide, RRID:SCR_000187)Statistical analysis: The data were analysed using Graph Pad Prism version 6 Software. Graph Pad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
-
