The impact of viral transport media on PCR assay results for the detection of nucleic acid from SARS-CoV-2
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Abstract
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SciScore for 10.1101/2020.06.09.142323: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
No key resources detected.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:However, these limitations are often not apparent to purchasing departments, especially during a pandemic, when any VTM may be mistakenly thought to be “fit for purpose”. In conclusion, the results of this study provide examples …
SciScore for 10.1101/2020.06.09.142323: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
No key resources detected.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:However, these limitations are often not apparent to purchasing departments, especially during a pandemic, when any VTM may be mistakenly thought to be “fit for purpose”. In conclusion, the results of this study provide examples of how the composition of a VTM could have an impact on the outcome of nucleic acid based testing and, in particular, situations where either there is a need to detect RNA that is not packaged into a nucleocapsid or where RNA constructs may be diluted in a VTM for use as a positive control in an assay or perhaps for proficiency testing. Finally, and particularly in the face of a pandemic, users should be reminded that products fit for one purpose may not be suitable for an alternative use. A product that may be eminently suitable for virus culture purposes could result in misleading results if used for nucleic acid-based tests.
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- No funding statement was detected.
- No protocol registration statement was detected.
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