Biodistribution and environmental safety of a live-attenuated YF17D-vectored SARS-CoV-2 vaccine candidate
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SciScore for 10.1101/2022.01.24.477505: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics IACUC: This project was approved by the KU Leuven ethical committee (P015-2020), following institutional guidelines approved by the Federation of European Laboratory Animal Science Associations (FELASA).
Euthanasia Agents: Hamsters were euthanized by intraperitoneal administration of 500 μL (hamsters) Dolethal (200 mg/mL sodium pentobarbital, Vétoquinol SA).Sex as a biological variable Biodistribution: WT hamsters (6-8 weeks old, female) were inoculated intraperitoneally with 104 PFU/mL dose of YF17D (n = 6) or YF-S0 (n = 6). Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Res… SciScore for 10.1101/2022.01.24.477505: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics IACUC: This project was approved by the KU Leuven ethical committee (P015-2020), following institutional guidelines approved by the Federation of European Laboratory Animal Science Associations (FELASA).
Euthanasia Agents: Hamsters were euthanized by intraperitoneal administration of 500 μL (hamsters) Dolethal (200 mg/mL sodium pentobarbital, Vétoquinol SA).Sex as a biological variable Biodistribution: WT hamsters (6-8 weeks old, female) were inoculated intraperitoneally with 104 PFU/mL dose of YF17D (n = 6) or YF-S0 (n = 6). Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources Original YF17D vaccine (Stamaril, Sanofi-Pasteur; lot number G5400) was purchased via the pharmacy of the University Hospital Leuven and passaged twice in Vero E6 cells prior to use. Vero E6suggested: RRID:CVCL_XD71)All virus stocks were titrated by plaque assay on BHK21 cells [17]. BHK21suggested: ATCC Cat# CRL-6282, RRID:CVCL_1914)Software and Algorithms Sentences Resources Animal experiment: Hamsters: Wild-type (WT) outbred specific pathogen-free Syrian hamsters (Mesocricetus auratus) were purchased from Janvier Laboratories, France. Janvier Laboratoriessuggested: NoneHalf-maximal serum neutralizing titers (SNT50) were determined by curve fitting in GraphPad Prism 8. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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