Digital PCR to quantify ChAdOx1 nCoV-19 copies in blood and tissues
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SciScore for 10.1101/2021.05.28.446155: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Consent: Donors provided written informed consent (Ethical approval: PV4780).
Euthanasia Agents: After 30 min mice were sacrificed by cervical dislocation and tissue samples were collected as described.Sex as a biological variable Mouse model: C57Bl/6 mice of both sexes were anesthetized by intraperitoneal injection of ketamine (120 μg/g BW) and xylazine (16 μg/g BW in saline, 10 μl/g BW) and 50 μl ChAdOx1 nCov-19 vaccine were intradermally injected into the dorsal region. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
Experimental Models: Organisms/Strains Sentences Resources Mouse model: C57Bl/6 mice of both sexes were anesthetized by intraperitoneal … SciScore for 10.1101/2021.05.28.446155: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Consent: Donors provided written informed consent (Ethical approval: PV4780).
Euthanasia Agents: After 30 min mice were sacrificed by cervical dislocation and tissue samples were collected as described.Sex as a biological variable Mouse model: C57Bl/6 mice of both sexes were anesthetized by intraperitoneal injection of ketamine (120 μg/g BW) and xylazine (16 μg/g BW in saline, 10 μl/g BW) and 50 μl ChAdOx1 nCov-19 vaccine were intradermally injected into the dorsal region. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
Experimental Models: Organisms/Strains Sentences Resources Mouse model: C57Bl/6 mice of both sexes were anesthetized by intraperitoneal injection of ketamine (120 μg/g BW) and xylazine (16 μg/g BW in saline, 10 μl/g BW) and 50 μl ChAdOx1 nCov-19 vaccine were intradermally injected into the dorsal region. C57Bl/6suggested: NoneSoftware and Algorithms Sentences Resources Design of primers and probe for digital PCR: PCR primers and probes were designed with Primer Express version 3.0.1 (Thermo Fisher). Primer Expresssuggested: (Primer Express, RRID:SCR_014326)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- No funding statement was detected.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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